論文

査読有り
2013年4月26日

Extracellular heat shock protein A9 is a novel interaction partner of podoplanin in oral squamous cell carcinoma cells

Biochemical and Biophysical Research Communications
  • Masayuki Tsuneki
  • ,
  • Satoshi Maruyama
  • ,
  • Manabu Yamazaki
  • ,
  • Bo Xu
  • ,
  • Ahmed Essa
  • ,
  • Tatsuya Abé
  • ,
  • Hamzah Babkair
  • ,
  • Jun Cheng
  • ,
  • Tadashi Yamamoto
  • ,
  • Takashi Saku

434
1
開始ページ
124
終了ページ
130
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2013.03.057

In previous studies, we have shown several lines of evidence that podoplanin (PDPN) plays an important role in cell adhesion via its association with extracellular components in neoplastic conditions, though there has been no trial to search for PDPN-interaction molecules in the extracellular milieu. To screen for those molecules, we performed proteomics-based analysis using liquid chromatography-tandem mass spectrometry followed by co-immunoprecipitation for PDPN in ZK-1, an oral squamous cell carcinoma (SCC) cell system whose cell membrane molecules were cross-linked with each other in their extracellular compartments, and we identified heat shock protein (HSP) A9 as one of the extracellular PDPN bound molecules. Effects of transient PDPN knockdown by siRNA in ZK-1 were also comparatively examined for cellular behaviors in terms of HSPA9 expression and secretion. Finally, HSPA9 expression modes were immunohistochemically visualized in oral SCC tissue specimens. HSPA9 was secreted from ZK-1 cells, and the expression and secretion levels of HSPA9 gene and protein were well coordinated with those of PDPN. Immunohistochemically, HSPA9 and PDPN were co-localized in ZK-1 cells and oral SCC foci, especially in the peripheral zone. In conclusion, the results indicate that HSPA9 secreted by oral SCC cells interacts with PDPN on their cell surface in an autocrine manner and regulates their growth and invasiveness. © 2013 Elsevier Inc.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2013.03.057
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23541579
ID情報
  • DOI : 10.1016/j.bbrc.2013.03.057
  • ISSN : 0006-291X
  • ISSN : 1090-2104
  • PubMed ID : 23541579
  • SCOPUS ID : 84876785521

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