We report on the Lu-Hf and Re-Os isotope systematics of a well-characterized suite of spinet and garnet pyroxenites from the Gfohl Unit of the Bohemian Massif (Czech Republic, Austria). Lu-Hf mineral isochrons of three pyroxenites yield undistinguishable values in the range of 336-338 Ma. Similarly, the slope of Re-Os regression for most samples yields an age of 327 31 Ma. These values overlap previously reported Sm-Nd ages on pyroxenites, eclogites and associated peridotites from the Gfohl Unit, suggesting contemporaneous evolution of all these HT-HP rocks. The whole-rock Hf isotopic compositions are highly variable with initial epsilon(Hf) values ranging from -6.4 to +66. Most samples show a negative correlation between bulk rock Sm/Hf and epsilon(Hf) and, when taking into account other characteristics (e.g., high Sr-87/Sr-86), this may be explained by the presence of recycled oceanic sediments in the source of the pyroxenite parental melts. A pyroxenite from Horni Kounice has decoupled Hf-Nd systematics with highly radiogenic initial epsilon(Hf) of + 66 for a given epsilon(Nd) of +7.8. This decoupling is consistent with the presence of a melt derived from a depleted mantle component with high Lu/Hf. Finally, one sample from Bevary plots close to the MORB field in Hf-Nd isotope space consistent with its previously proposed origin as metamorphosed oceanic gabbro. Some of the websterites and thin layered pyroxenites have variable, but high Os concentrations paralleled by low initial gamma Os. This reflects the interaction of the parental pyroxenitic melts with a depleted peridotite wall rock. In turn, the radiogenic Os isotope compositions observed in most pyroxenite samples is best explained by mixing between unradiogenic Os derived from peridotites and a low-Os sedimentary precursor with highly radiogenic Os-187/Os-188. Steep increase of Os-187/Os-188 at nearly uniform Re-187/Os-188 found in a few pyroxenites may be connected with the absence of primary sulfides, but the presence of minor late stage sulfide-bearing veinlets likely associated with HT-HP metamorphism at crustal conditions. (C) 2016 Elsevier B.V. All rights reserved.