2010年4月
Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification
ENZYME AND MICROBIAL TECHNOLOGY
- ,
- ,
- 巻
- 46
- 号
- 5
- 開始ページ
- 391
- 終了ページ
- 396
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.enzmictec.2009.12.009
- 出版者・発行元
- ELSEVIER SCIENCE INC
Cereulide is the cyclic dodecadepsipeptide responsible for the emetic-type food-borne disease caused by Bacillus cereus. It is synthesized enzymatically by non-ribosomal peptide synthetases. In this study, we established an RNA-specific amplification assay for the detection of mRNA transcribed from the cesA gene, one of the 7 genes constituting the cereulide synthetase (ces) gene cluster of emetic-type B. cereus. The assay detected as few as 1000 copies of this RNA. Examination of the total RNAs extracted from 7 strains of cereulide-producing B. cereus, 6 strains of cereulide non-producing B. cereus, 6 strains of other Bacillus species, and 10 non-Bacillus strains showed that the assay specifically detected emetic-type B. cereus strains. RNA-specific amplification using RNA extracts provided comparable results to RT-PCR assays using RNA extracts and PCR assays using DNA extracts. The RNA-specific amplification assay is suitable for the detection of mRNA transcribed from the cesA gene. (C) 2009 Elsevier Inc. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.enzmictec.2009.12.009
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201002262437508167
- CiNii Articles
- http://ci.nii.ac.jp/naid/120002191870
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000275539400010&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.enzmictec.2009.12.009
- ISSN : 0141-0229
- J-Global ID : 201002262437508167
- CiNii Articles ID : 120002191870
- Web of Science ID : WOS:000275539400010