2014年3月
A Chemical Probe that Labels Human Pluripotent Stem Cells
CELL REPORTS
- 巻
- 6
- 号
- 6
- 開始ページ
- 1165
- 終了ページ
- 1174
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.celrep.2014.02.006
- 出版者・発行元
- CELL PRESS
A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.celrep.2014.02.006
- ISSN : 2211-1247
- Web of Science ID : WOS:000333465000020