2017年4月
Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp JSC4
SCIENTIFIC REPORTS
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- 巻
- 7
- 号
- 7
- 開始ページ
- 45471
- 終了ページ
- 45471
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep45471
- 出版者・発行元
- NATURE PUBLISHING GROUP
Biodiesel production using microalgae would play a pivotal role in satisfying future global energy demands. Understanding of lipid metabolism in microalgae is important to isolate oleaginous strain capable of overproducing lipids. It has been reported that reducing starch biosynthesis can enhance lipid accumulation. However, the metabolic mechanism controlling carbon partitioning from starch to lipids in microalgae remains unclear, thus complicating the genetic engineering of algal strains. We here used "dynamic" metabolic profiling and essential transcription analysis of the oleaginous green alga Chlamydomonas sp. JSC4 for the first time to demonstrate the switching mechanisms from starch to lipid synthesis using salinity as a regulator, and identified the metabolic rate-limiting step for enhancing lipid accumulation (e. g., pyruvate-to-acetyl-CoA). These results, showing salinity-induced starch-tolipid biosynthesis, will help increase our understanding of dynamic carbon partitioning in oleaginous microalgae. Moreover, we successfully determined the changes of several key lipid-synthesis- related genes (e. g., acetyl-CoA carboxylase, pyruvate decarboxylase, acetaldehyde dehydrogenase, acetylCoA synthetase and pyruvate ferredoxin oxidoreductase) and starch-degradation related genes (e. g., starch phosphorylases), which could provide a breakthrough in the marine microalgal production of biodiesel.
- リンク情報
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- DOI
- https://doi.org/10.1038/srep45471
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28374798
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379629
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000398166300001&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1038/srep45471
- ISSN : 2045-2322
- PubMed ID : 28374798
- PubMed Central 記事ID : PMC5379629
- Web of Science ID : WOS:000398166300001