Jun, 2011
Mechanical stretch increases CCN2/CTGF expression in anterior cruciate ligament-derived cells
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- Volume
- 409
- Number
- 2
- First page
- 247
- Last page
- 252
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.bbrc.2011.04.138
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Anterior cruciate ligament (ACL)-to-bone interface serves to minimize the stress concentrations that would arise between two different tissues. Mechanical stretch plays an important role in maintaining cell-specific features by inducing CCN family 2/connective tissue growth factor (CCN2/CTGF). We previously reported that cyclic tensile strain (CTS) stimulates alpha 1(I) collagen (COL1A1) expression in human ACL-derived cells. However, the biological function and stress-related response of CCN2/CTGF were still unclear in ACL fibroblasts. In the present study, CCN2/CTGF was observed in ACL-to-bone interface, but was not in the midsubstance region by immunohistochemical analyses. CTS treatments induced higher increase of CCN2/CTGF expression and secretion in interface cells compared with midsubstance cells. COL1A1 expression was not influenced by CCN2/CTGF treatment in interface cells despite CCN2/CTGF stimulated COL1A1 expression in midsubstance cells. However, CCN2/CTGF stimulated the proliferation of interface cells. Our results suggest that distinct biological function of stretch-induced CCN2/CTGF might regulate region-specific phenotypes of ACL-derived cells. (C) 2011 Elsevier Inc. All rights reserved.
- Link information
- ID information
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- DOI : 10.1016/j.bbrc.2011.04.138
- ISSN : 0006-291X
- Pubmed ID : 21569762
- Web of Science ID : WOS:000291779100017