2018年6月21日
Effects of combined use of recombinant human Fibroblast growth factor-2 and β-Tricalcium phosphate on ridge preservation in dehiscence bone defects after tooth extraction: A split-mouth study in dogs
Europerio9
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- 記述言語
- 英語
- 会議種別
- ポスター発表
- 主催者
- The European Federation of Periodontology
- 開催地
- Amsterdam, Netherlands
Title
Effects of combined use of recombinant human Fibroblast growth factor-2 and β-Tricalcium phosphate on ridge preservation in dehiscence bone defects after tooth extraction: A split-mouth study in dogs.
Background & Aim
Tooth extraction often leads to an alveolar ridge of extremely reduced height and width, causing esthetic and functional disorder. Various bone graft materials have been used for alveolar ridge preservation. Anzai and co-workers reported periodontal regeneration using recombinant human fibroblast growth factor-2 (rhFGF-2) with β-Tricalcium phosphate (β-TCP) in beagle dog one-wall periodontal defects. This therapeutic combination was shown to improve radiographical and histological parameters as compared with β-TCP alone. There is still a lack of data regarding the effects of rhFGF-2, either alone or with a bone graft, on ridge preservation after tooth extraction.
This study was aimed to evaluate effects of concomitant use of rhFGF-2 with β-TCP on ridge preservation in a dehiscence defect model after tooth extraction in dogs.
Methods
The first premolars of six beagle dogs were extracted after removing the buccal bone, and bone defects of 4×4×5mm (mesio-distal×bucco-palatal×depth) were created. Bilateral defects were filled with β-TCP (Osferion®) mixed with 0.3% (w/v) rhFGF-2 (test sites) or the scaffold alone (control sites). Twelve weeks after the operation, micro-computed tomographical (micro-CT) and histological evaluations were performed.
Results
There were no signs of clinical inflammation at twelve weeks after surgery. Micro-CT measurements revealed a significantly greater preserved bone volume at test sites (42.6±0.88mm3) as compared with control sites (34.3±0.81mm3 p=0.005). Histologically, resorption of β-TCP particles was observed in both groups. New bone formation was larger in the test group as compared with the control group.
Conclusions
Within the limitation of this study, combined use of rhFGF-2 and β-TCP may be an effective treatment modality for alveolar ridge preservation in fresh extraction sockets with buccal bone defects.
Effects of combined use of recombinant human Fibroblast growth factor-2 and β-Tricalcium phosphate on ridge preservation in dehiscence bone defects after tooth extraction: A split-mouth study in dogs.
Background & Aim
Tooth extraction often leads to an alveolar ridge of extremely reduced height and width, causing esthetic and functional disorder. Various bone graft materials have been used for alveolar ridge preservation. Anzai and co-workers reported periodontal regeneration using recombinant human fibroblast growth factor-2 (rhFGF-2) with β-Tricalcium phosphate (β-TCP) in beagle dog one-wall periodontal defects. This therapeutic combination was shown to improve radiographical and histological parameters as compared with β-TCP alone. There is still a lack of data regarding the effects of rhFGF-2, either alone or with a bone graft, on ridge preservation after tooth extraction.
This study was aimed to evaluate effects of concomitant use of rhFGF-2 with β-TCP on ridge preservation in a dehiscence defect model after tooth extraction in dogs.
Methods
The first premolars of six beagle dogs were extracted after removing the buccal bone, and bone defects of 4×4×5mm (mesio-distal×bucco-palatal×depth) were created. Bilateral defects were filled with β-TCP (Osferion®) mixed with 0.3% (w/v) rhFGF-2 (test sites) or the scaffold alone (control sites). Twelve weeks after the operation, micro-computed tomographical (micro-CT) and histological evaluations were performed.
Results
There were no signs of clinical inflammation at twelve weeks after surgery. Micro-CT measurements revealed a significantly greater preserved bone volume at test sites (42.6±0.88mm3) as compared with control sites (34.3±0.81mm3 p=0.005). Histologically, resorption of β-TCP particles was observed in both groups. New bone formation was larger in the test group as compared with the control group.
Conclusions
Within the limitation of this study, combined use of rhFGF-2 and β-TCP may be an effective treatment modality for alveolar ridge preservation in fresh extraction sockets with buccal bone defects.