2008年10月
Involvement of Voltage-Gated Sodium Channel Na(v)1.8 in the Regulation of the Release and Synthesis of Substance P in Adult Mouse Dorsal Root Ganglion Neurons
JOURNAL OF PHARMACOLOGICAL SCIENCES
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- 巻
- 108
- 号
- 2
- 開始ページ
- 190
- 終了ページ
- 197
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1254/jphs.08163FP
- 出版者・発行元
- JAPANESE PHARMACOLOGICAL SOC
This study was conducted to determine whether Na(v)1.8 contributes to the release and/or synthesis of substance P (SP) in adult mice dorsal root ganglion (DRG) neurons. The SP released from cultured DRG neurons of Nav1.8 knock-out mice exposed to either capsaicin or KCI was significantly lower than that from wild-type (C57BL/6) mice based on a radioimmunoassay. The SP level of L6 DRG in Nav1.8 knock-out mice was also lower than that in wild-type mice. After chronic constriction injury (CCI) of the sciatic nerve, the level of SP decreased in the L6 ipsilateral DRG of wild-type but not Na(v)1.8 knock-out mice. The preprotachykinin-A (PPT-A) mRNAs in L4-6 DRGs of Nav1.8 knock-out mice also fell to half their normally abundant levels of expression. There were significant increases in Na(v)1.8 expression of the L6 contralateral DRG from wild-type mice and in the percentage of neurons expressing neurokinin-1 receptor in the cytosol of L6 DRGs from wild-type or Na(v)1.8 knock-out mice. These findings suggest that Na(v)1.8 is involved in the regulation of the release and synthesis of SP in the DRG neurons of wild-type mice.
- リンク情報
- ID情報
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- DOI : 10.1254/jphs.08163FP
- ISSN : 1347-8613
- PubMed ID : 18845912
- Web of Science ID : WOS:000260590100006