論文

査読有り
2017年3月

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Nature biotechnology
  • Shimatani, Zenpei
  • ,
  • Kashojiya, Sachiko
  • ,
  • Takayama, Mariko
  • ,
  • Terada, Rie
  • ,
  • Arazoe, Takayuki
  • ,
  • Ishii, Hisaki
  • ,
  • Teramura, Hiroshi
  • ,
  • Yamamoto, Tsuyoshi
  • ,
  • Komatsu, Hiroki
  • ,
  • Miura, Kenji
  • ,
  • Ezura, Hiroshi
  • ,
  • Nishida, Keiji
  • ,
  • Ariizumi, Tohru
  • ,
  • Kondo, Akihiko

35
5
開始ページ
441
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/nbt.3833
出版者・発行元
NATURE PUBLISHING GROUP

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.

リンク情報
DOI
https://doi.org/10.1038/nbt.3833

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