2017年3月
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Nature biotechnology
- 巻
- 35
- 号
- 5
- 開始ページ
- 441
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/nbt.3833
- 出版者・発行元
- NATURE PUBLISHING GROUP
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
- リンク情報
- ID情報
-
- DOI : 10.1038/nbt.3833
- ISSN : 1546-1696