Papers

Peer-reviewed Lead author
Jul, 2011

Comparison of piggyBac transposition efficiency between linear and circular donor vectors in mammalian cells

JOURNAL OF BIOTECHNOLOGY
  • Hideyuki Nakanishi
    • ,
  • Yuriko Higuchi
    • ,
  • Shigeru Kawakami
    • ,
  • Fumiyoshi Yamashita
    • ,
  • Mitsuru Hashida
Volume
154
Number
4
First page
205
Last page
208
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.jbiotec.2011.05.009
Publisher
ELSEVIER SCIENCE BV
The piggyBac transposon has recently attracted attention as a tool for transgene integration in mammalian cells. However, previous studies involving piggyBac investigated only transposition from circular DNA, although some linear DNA vectors are used to transfect mammalian cells. In this study, we compared the transposition efficiency of piggyBac between linear and circular DNA. Colony counting assay, luciferase assay, and plasmid rescue assay showed that piggyBac transposon can transpose from linear DNA, but its efficiency is lower than the transposition efficiency from circular DNA. These results suggest that circular DNA is more suitable as donor vectors of piggyBac than linear DNA. (C) 2011 Elsevier B. V. All rights reserved.
Link information
DOI
https://doi.org/10.1016/j.jbiotec.2011.05.009
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21640767
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000292958600002&DestApp=WOS_CPL
ID information
  • DOI : 10.1016/j.jbiotec.2011.05.009
  • ISSN : 0168-1656
  • eISSN : 1873-4863
  • Pubmed ID : 21640767
  • Web of Science ID : WOS:000292958600002
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