This paper describes DNA logic gates with biological nanopores which are formed by membrane proteins constituted in lipid bilayer membranes (BLMs). BLMs as artificial membrane are formed in droplet-contact method (DCM). Split- and-contact method (SCM) is the system applied DCM. BLMs are simply and speedy formed in SCM. And we can use the rapture of BLMs as positive manner (ZAP) because SCM can allow ruptured BLMs to be re-formed many times. Biological molecules are translocated into the nanopores which are formed by aHL constituted in BLMs using SCM. We focus on DNA as one of the biological molecules. Single-strand DNA (ssDNA) is translocated into the nanopore, while disturbing the translocation of ions and Double-strand DNA (dsDNA) blocks the nanopore. In this study, we demonstrated DNA-OR gate and DNA-NOT gate as DNA logic gates in these system, SCM and the characteristic of DNA.