1995年12月
Production of eicosapentaenoic acid by Saprolegnia sp 28YTF-1
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
- ,
- 巻
- 72
- 号
- 12
- 開始ページ
- 1545
- 終了ページ
- 1549
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/BF02577852
- 出版者・発行元
- AMER OIL CHEMISTS SOC
Saprolegnia sp. 28YTF-1, isolated from a freshwater sample, is a potent producer of 5,8,11,14,17-cis-eicosapentaenoic acid (EPA). The fungus used various kinds of carbon sources, such as starch, dextrin, sucrose, glucose, and olive oil for growth, and olive oil was the best carbon source for EPA production. The EPA content reached 17 mg/g dry mycelium (0.25 mg/L) when the fungus was grown in a medium that contained 2.5% olive oil and 0.5% yeast extract, at PH 6.0 and 28 degrees C for 6 d with shaking. Accompanying production of arachidonic acid (AA; 3.2 mg/g dry mycelia, EPA/AA = 5.1) and other omega 6 polyunsaturated fatty acids was low. Both EPA content and EPA/AA ratio increased in parallel by lowering growth temperature. Triglyceride was the major mycelial lipid (ca. 84%), but EPA comprised only 2.2% of the total fatty acids of this lipid. About 40% of the EPA produced was found in polar lipids, such as phosphatidylethanolamine (EPA content, 28.2%), phosphatidylcholine (13.6%), and phosphatidylserine (21.2%).
- リンク情報
- ID情報
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- DOI : 10.1007/BF02577852
- ISSN : 0003-021X
- CiNii Articles ID : 30026723856
- Web of Science ID : WOS:A1995TK89200024