Jun, 2012
Development of real-time radioisotope imaging systems for plant nutrient uptake studies
Philosophical Transactions of The Royal Society B-Biological Sciences
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- Volume
- 367
- Number
- 1595
- First page
- 1501
- Last page
- 1508
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1098/rstb.2011.0229
- Publisher
- ROYAL SOC
Ionic nutrition is essential for plant development. Many techniques have been developed to image and (or) measure ionic movement in plants. Nevertheless, most of them are destructive and limit the analysis. Here, we present the development of radioisotope imaging techniques that overcome such restrictions and allow for real-time imaging of ionic movement. The first system, called macroimaging, was developed to visualize and measure ion uptake and translocation between organs at a whole-plant scale. Such a device is fully compatible with illumination of the sample. We also modified fluorescent microscopes to set up various solutions for ion uptake analysis at the microscopic level. Both systems allow numerical analysis of images and possess a wide dynamic range of detection because they are based on radioactivity.
- Link information
- ID information
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- DOI : 10.1098/rstb.2011.0229
- ISSN : 0962-8436
- eISSN : 1471-2970
- Pubmed ID : 22527392
- Web of Science ID : WOS:000303107900007