Tomoko Nakanishi

Profile Information

Affiliation: Assistant Professor, Juntendo University

Degree: Doctor(Mar, 2002, Osaka University)

Researcher number: 10344863
J-GLOBAL ID: 200901002307764483
researchmap Member ID: 5000043869

Research Interests

Research Areas

Research History

Jun, 2023 - Present

Associate professor, Center for Biomedical Research Resources, Juntendo University Graduate School of Medicine, Juntendo University
Apr, 2021 - May, 2023

Center for Biomedical Research Resources, Juntendo University Graduate School of Medicine, Juntendo University
Apr, 2017 - Mar, 2021

Laboratory of Virology, Institute of Microbial Chemistry (BIKAKEN), Microbial Chemistry Foundation
Nov, 2015 - Mar, 2017

Laboratory of Molecular Genetics, Institute of Medical Science, The University of Tokyo
Mar, 2014 - Oct, 2015

The Institute of Medical Science, The University of Tokyo
Dec, 2006 - Feb, 2014

Faculty of Medicine, Tottori University
May, 2002 - Nov, 2006

Graduate School of Life and Environmental Sciences, University of Tsukuba
Apr, 1999 - Mar, 2002

DC1, 特別研究員, 日本学術振興会
1999 - 2002

日本学術振興会特別研究員DCI
2002

大阪大学遺伝情報実験センター研究員

Education

Apr, 1999 - Mar, 2002

Osaka University
Apr, 1997 - Mar, 1999

Graduate School, Division of Pharmaceutical Sciences, Osaka University
Apr, 1993 - Mar, 1997

School of Pharmaceutical Sciences, Osaka University

Awards

May, 2013

優秀演題賞, 発光イメージングを利用したマウスにおけるリアルタイム骨形成活性評価, 日本骨代謝学会

中西友子
Apr, 2013

ANSBMS 2013 Travel award, 発光イメージングを利用したマウスにおけるリアルタイム骨形成活性評価, 日本骨代謝学会

中西友子
Nov, 2003

学会賞, キメラマウスを用いた精子の子宮・卵管移行メカニズムの解析, 日本疾患モデル学会

中西友子

Papers

Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector.

Satoko Kise, Ayano Iijima, Chika Nagao, Tadashi Okada, Miyu Nishikawa, Shinichi Ikushiro, Tomoko Nakanishi, Shigeto Sato, Kaori Yasuda, Toshiyuki Sakaki

Scientific reports, 13(1) 18528-18528, Oct 28, 2023
Functional analysis of vitamin D receptor (VDR) using adenovirus vector

Satoko Kise, Ayano Iijima, Chika Nagao, Tadashi Okada, Hiroki Mano, Miyu Nishikawa, Shinichi Ikushiro, Yoshiaki Kanemoto, Shigeaki Kato, Tomoko Nakanishi …

The Journal of Steroid Biochemistry and Molecular Biology, 230 106275-106275, Jun, 2023 Peer-reviewed
Expression of Rat Cyp27b1 in HepG2 Cells Using Adenovirus Vector and Its Application to Evaluation of Self-Made and Commercially Available Anti-Cyp27b1 Antibodies.

Chika Nagao, Satoko Kise, Ayano Iijima, Tadashi Okada, Tomoko Nakanishi, Shigeto Sato, Miyu Nishikawa, Shinchi Ikushiro, Kaori Yasuda, Toshiyuki Sakaki

Journal of nutritional science and vitaminology, 69(2) 90-97, 2023
Adenovirus Vectors Expressing Eight Multiplex Guide RNAs of CRISPR/Cas9 Efficiently Disrupted Diverse Hepatitis B Virus Gene Derived from Heterogeneous Patient

Yuya Kato, Hirotaka Tabata, Kumiko Sato, Mariko Nakamura, Izumu Saito, Tomoko Nakanishi

Int. J. Mol. Sci., 22 10570, Oct, 2021 Peer-reviewedLast author
Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing.

Tomoko Nakanishi, Aya Maekawa, Mariko Suzuki, Hirotaka Tabata, Kumiko Sato, Mai Mori, Izumu Saito

Scientific reports, 11(1) 3961-3961, Feb 17, 2021 Peer-reviewedCorresponding author
Highly multiplex guide RNA expression units of CRISPR/Cas9 were completely stable using cosmid amplification in a novel polygonal structure

Tomoko Nakanishi, Aya Maekawa, Hirotaka Tabata, Takashi Yoshioka, Zheng Pei, Kumiko Sato, Mai Mori, Masaya Kato, Izumu Saito

JOURNAL OF GENE MEDICINE, 21(11) e3115, Nov, 2019 Peer-reviewed
Insulin and osteocalcin: further evidence for a mutual cross-talk

Francesco L. Bilotta, Biagio Arcidiacono, Sebastiano Messineo, Marta Greco, Eusebio Chiefari, Domenico Britti, Tomoko Nakanishi, Daniela P. Foti, Antonio Brunetti

Endocrine, 59(3) 622-632, Mar, 2018
Fate of methylated/unmethylated H19 imprinting control region after paternal and maternal pronuclear injection

Asami Oji, Tomojiro Amano, Yasuaki Maeta, Naohiro Hori, Kiyotaka Hatsuzawa, Kenzo Sato, Tomoko Nakanishi

EXPERIMENTAL ANIMALS, 66(4) 367-378, Oct, 2017 Peer-reviewedLast authorCorresponding author
Histone macroH2A1.2 promotes metabolic health and leanness by inhibiting adipogenesis

Valerio Pazienza, Concetta Panebianco, Francesca Rappa, Domenico Memoli, Michela Borghesan, Sara Cannito, Asami Oji, Giuseppe Mazza, Domenico Tamburrino, Giuseppe Fusai …

EPIGENETICS & CHROMATIN, 9 45, Oct, 2016 Peer-reviewed
SIRT1-metabolite binding histone macroH2A1.1 protects hepatocytes against lipid accumulation

Valerio Pazienza, Michela Borghesan, Tommaso Mazza, Fareeba Sheedfar, Concetta Panebianco, Roger Williams, Gianluigi Mazzoccoli, Angelo Andriulli, Tomoko Nakanishi, Manlio Vinciguerra

AGING-US, 6(1) 35-47, Jan, 2014 Peer-reviewed
Visualization of inactive X chromosome in preimplantation embryos utilizing MacroH2AEGFP transgenic mouse

Atsumi Soma, Kenzo Sato, Tomoko Nakanishi

GENESIS, 51(4) 259-267, Apr, 2013 Peer-reviewedLast authorCorresponding author
Identification of the Control Region of Pancreatic Expression of Bmp4 In Vitro and In Vivo

Mayu Yasunaga, Eiji Masui, Asami Oji, Atsumi Soma, Mitsuhiko Osaki, Tomoko Nakanishi, Kenzo Sato

PLOS ONE, 8(4) e61821, Apr, 2013 Peer-reviewed
In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene

Tomoko Nakanishi, Rumiko Saito, Makoto Taniguchi, Haruka Oda, Atsumi Soma, Mayu Yasunaga, Mariko Yamane, Kenzo Sato

BIOMED RESEARCH INTERNATIONAL, 2013 895706, 2013 Peer-reviewed
Bioluminescence imaging of bone formation using hairless osteocalcin-luciferase transgenic mice

Tomoko Nakanishi, Kazuo Kokubun, Haruka Oda, Mika Aoki, Atsumi Soma, Makoto Taniguchi, Yasuhiro Kazuki, Mitsuo Oshimura, Kenzo Sato

BONE, 51(3) 369-375, Sep, 2012 Peer-reviewed
A crucial role of bone morphogenetic protein signaling in the wound healing response in acute liver injury induced by carbon tetrachloride.

Oumi N, Taniguchi KA, Kanai AM, Yasunaga M, Nakanishi T, Sato K

International journal of hepatology, 2012 476820, 2012 Peer-reviewed
Establishment and Characterization of a Transgenic Mouse Model for In Vivo Imaging of Bmp4 Expression in the Pancreas

Mayu Yasunaga, Nao Oumi, Mitsuhiko Osaki, Yasuhiro Kazuki, Tomoko Nakanishi, Mitsuo Oshimura, Kenzo Sato

PLOS ONE, 6(9) e24956, Sep, 2011 Peer-reviewed
Development of evaluation system for bioactive substances using human artificial chromosome-mediated osteocalcin gene expression

Yusuke Takahashi, Saori Tsuji, Yasuhiro Kazuki, Makoto Noguchi, Ichiro Arifuku, Yukihiro Umebayashi, Tomoko Nakanishi, Mitsuo Oshimura, Kenzo Sato

JOURNAL OF BIOCHEMISTRY, 148(1) 29-34, Jul, 2010 Peer-reviewed
Functional Roles of Mouse Sperm Hyaluronidases, HYAL5 and SPAM1, in Fertilization

Masanori Kimura, Ekyune Kim, Woojin Kang, Misuzu Yamashita, Mariko Saigo, Taiga Yamazaki, Tomoko Nakanishi, Shin-ichi Kashiwabara, Tadashi Baba

BIOLOGY OF REPRODUCTION, 81(5) 939-947, Nov, 2009 Peer-reviewed
Birth of normal offspring from mouse eggs activated by a phospholipase C zeta protein lacking three EF-hand domains

Tomoko Nakanishi, Naoko Ishibashi, Haruka Kubota, Kimiko Inoue, Narumi Ogonuki, Atsuo Ogura, Shin-ichi Kashiwabara, Tadashi Baba

JOURNAL OF REPRODUCTION AND DEVELOPMENT, 54(4) 244-249, Aug, 2008 Peer-reviewed
Non-canonical poly(A) polymerase in mammalian gametogenesis.

Kashiwabara S, Nakanishi T, Kimura M, Baba T

Biochimica et biophysica acta, 1779(4) 230-238, Apr, 2008 Peer-reviewed

Books and Other Publications

「ウイルスベクターとゲノム編集」実験医学別冊

前川文, 中西友子, 斎藤泉, 担当:共著

羊土社, Dec, 2016 (ISBN: 9784758101981)

Presentations

高効率かつ安全な超多重ガイドRNA発現アデノベクターの開発と供給：がん、肝炎、新型コロナ、オートファジー、遺伝病のゲノム編集治療研究への応用

中西友子, 中原知美, 山口貴世志, 古川洋一, 福原崇介, 茶山弘美, 森石恆司, 神谷亘, 一村義信, 小松雅明, 服部信孝, 斎藤泉, 清野透

第29回日本遺伝子細胞治療学会, Sep 13, 2023
初心者でも安心！ゼロから始めるアデノベクター～ゲノム編集治療に向けた挑戦

中西友子, 山地恵, 中村眞理子, 佐藤栄人, 斎藤泉

第70回日本実験動物学会, May 25, 2023 Invited
CRISPR/Cas9システムによるゲノム編集アデノベクターの開発とフェニルケトン尿症マウス治療モデルの構築

中西友子, 中村眞理子, 山地恵, 大庭俊一, 大石智一, 原川晃子, 川田学, 斎藤泉

第69回日本実験動物学会, May 19, 2022
Development of AdVs for CRISPR/Cas9-based gene editing and its application to PKU mouse model

Tomoko Nakanishi, Tabata Hirotaka, Tadashi Okada, Ryoko Fuse, Mariko Nakamura, Tomokazu Ohishi, Syunichi Ohba, Akiko Harakawa, Manabu Kawada, Izumu Saito

May 23, 2020
Development of all-in-one type adenovirus vectors simultaneously expressing eight multiplex double-nicking guide RNAs and Cas9-nickase

Sep 27, 2019
All-in-one adenovirus vectors expressing both highly multiplex double-nicking guide RNAs and Cas9 nickase for genome editing therapy

NAKANISHI Tomoko, Hirotaka Tabata, Mariko Nakamura, Ryoko Fuse, Izumu Saito

The 25th Annual Meeting of Japan Society of Gene and Cell Therapy, Jul 22, 2019
Adenovirus vectors expressing highly-multiplex double-nicking guide RNAs in vivo: high efficiency and very low off-target

NAKANISHI Tomoko

Sep 27, 2018
Development of adenovirus vectors expressing highly multiplex double-nicking guide RNAs for in vivo genome editing aiming clinical application

NAKANISHI Tomoko

Jul 27, 2018
Development of adenovectors expressing multiplex gRNAs and its application to in vivo genome editing

NAKANISHI Tomoko

Jun 20, 2018
Utilization of humanized mice as a small animal model for measles virus infection.

NAKANISHI Tomoko

International unin o microbiological societies 2017, Jul 17, 2017
免疫系ヒト化マウスを用いた麻疹ウイルスの小動物感染モデル

中西友子

第64回日本実験動物学会, May 26, 2017
遺伝子改変・ゲノム編集を用いたモデルマウスの作製とヒト疾患への応用

中西友子

公益財団法人微生物化学研究所所内セミナー, Dec 7, 2016
Establishment of measles virus infection model utilizing humanized mouse.

NAKANISHI Tomoko

Oct 26, 2016
Establishment of measles virus infection model utilizing humanized mouse.

NAKANISHI Tomoko

Oct 24, 2016
Establishment of MHC class II knockout NSG mice utilizing CRISPR/Cas9 system.

NAKANISHI Tomoko

Sep 6, 2016
免疫ヒト化マウスを利用した麻疹ウイルス感染モデルの構築

中西友子

第63回日本実験動物学会, May 20, 2016
発光イメージングを利用したマウスにおけるリアルタイム骨形成活性評価

中西友子

第8回 Bone research seminar, Feb, 2014 Invited

Teaching Experience

Professional Memberships

Research Projects

二本鎖切断を伴わないノックインアデノベクターによる新規遺伝子治療モデルの開発

科学研究費助成事業, 基盤研究(B), 日本学術振興会, Apr, 2023 - Mar, 2027

中西友子, 古川洋一
遺伝子疾患動物評価系を用いたビタミンD誘導体の開発および遺伝子治療法の確立

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2022 - Mar, 2025
Development of genome-editing drugs of adenovirus vector expressing eight multiplex guide RNAs using models of HBV replication in humanized mice and of HBV carcinomas

AMED, Apr, 2022 - Mar, 2025
超多重ガイドRNA/Cas9 nickase搭載一体型アデノウイルスベクターを用いたパピローマウイルス感染病変のゲノム編集治療法の開発

AMED, Apr, 2022 - Mar, 2024
Drugs of Adenovirus vectors expressing eight multiplex shRNAs and guide RNAs aiming control and cure of COVID-19

AMED, Jul, 2022 - Mar, 2023

Wataru Kamitani, Takasuke Fukuhara, Koji Moriishi, Tomoko Nakanishi
ゲノム編集用一体型アデノベクターとヒト型マウスの開発による遺伝子治療モデルの構築

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2019 - Mar, 2022

中西友子, 斎藤泉
効率的なHBV増殖細胞系、動物モデル系の確立とそれらを用いたHBV治療法開発

感染症実用化研究事業肝炎等克服実用化研究事業Ｂ型肝炎創薬実用化等研究事業, 日本医療研究開発機構, Apr, 2020 - Mar, 2022

藤田尚志, 松浦善治, 土方誠, 加藤宣之, 中西友子, 竹原徹郎, 柘植雅貴, 水腰英四郎, 五十川正記
Development of genome-editing based therapy of papillomavirus-infected lesions with all-in-one adenovirus vectors expressing super-multiplex guide RNA

AMED, Apr, 2019 - Mar, 2022
Development of anti-COVID-19 therapy to suppress severe pneumonia by combination using adenovirus vector with ultra-multi guide RNA expression system and protease inhibitors.

Jul, 2020 - Mar, 2021
安全かつ高効率なゲノム編集治療をめざした単一型多重ガイドRNA発現アデノベクターの開発

革新的医療技術創出拠点プロジェクト（橋渡し研究戦略的推進プログラム）, 日本医療研究開発機構（AMED）, Apr, 2018 - Mar, 2020

斎藤泉、中西友子
Establishment of humanized mice as a small animal model for measles virus infection

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2019

NAKANISHI Tomoko, KAI Chieko
HBVゲノムを多数同時切断し破壊する多重ガイドRNA発現アデノベクター及びモニター用蛍光誘導HBVゲノムの開発

肝炎等克服実用化研究事業, 日本医療研究開発機構（AMED）, Apr, 2016 - Mar, 2019

斎藤泉、山﨑学、中西友子
HBVゲノムを多数同時切断し破壊する多重ガイドRNA発現アデノベクター及びモニター用蛍光誘導HBVゲノムの開発

肝炎等克服実用化研究事業, 日本医療研究開発機構（AMED）, Apr, 2016 - Mar, 2017

松浦善治
Analysis of bone formation activity during pregnancy by in vivo imaging

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2013 - Mar, 2016

NAKANISHI Tomoko, HATSUZAWA Kiyotaka
外来性DNAを用いた雌雄ゲノムにおけるインプリント領域制御機構の解析

科学研究費助成事業特定領域研究, 特定領域研究, 日本学術振興会, Apr, 2011 - Mar, 2013

中西友子
Analysis of difference between female and male genome determined in pronuclear stage embryo.

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, 2010 - 2012

NAKANISHI Tomoko, SOMA Atsumi, OJI Asami
BMP受容体欠損マウスにおける肝細胞の損傷修復機構

基盤研究(C), Apr, 2008 - Mar, 2011

佐藤建三
X染色体不活性化におけるレトロポゾンを介したRNAネットワーク

科学研究費助成事業特定領域研究, 特定領域研究, 日本学術振興会, 2009 - 2010

中西友子
雌性生殖器での精子機能制御の仕組み

基盤研究(A), Apr, 2006 - Mar, 2009

馬場忠
Analysis of genomic reprogramming in mammals by utilizing inactive X chromosome.

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, 2008 - 2009

NAKANISHI Tomoko

Industrial Property Rights

特願2023-014899 HPV陽性がん又はHPV陽性前がん病変遺伝子治療用医薬組成物、及び組換えアデノウイルスベクター

清野透, 中原知美, 中西友子, 斎藤泉
特願2019-121668 新規ウイルスベクターおよびその製造方法と使用方法

斎藤泉, 中西友子
PCT/JP2019/037255 新規ウイルスベクターおよびその製造方法と使用方法

斎藤泉, 中西友子

Media Coverage

ゲノム編集切る場所誤らず

日経産業新聞, Aug 14, 2019 Newspaper, magazine

Profile Information

Research Interests

Research Areas

Research History

Education

Awards

Papers

Books and Other Publications

Presentations

Teaching Experience

Professional Memberships

Research Projects

Industrial Property Rights

Media Coverage

Menu

Coauthors