2006年9月
Gene structure and regulation of alkane monooxygenases in propane-utilizing Mycobacterium sp TY-6 and Pseudonocardia sp TY-7
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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- 巻
- 102
- 号
- 3
- 開始ページ
- 184
- 終了ページ
- 192
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1263/jbb.102.184
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7 were isolated from soil samples as propane-utilizing bacteria and were found to be able to utilize various gaseous and liquid n-alkanes as carbon and energy sources. One gene cluster, M-prmABCD, and two gene clusters, P-prm1ABCD and P-prm2ABCD, were cloned from the genomes of Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7, respectively. These gene clusters are homologous to the gene cluster encoding the multicomponent propane monooxygenase (prmABCD) of Gordonia sp. TY-5. The expression of prm gene clusters in Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7 was shown to be induced by gaseous n-alkanes (C-2-C-4) except methane, suggesting that the products of these genes are involved in gaseous n-alkane oxidation. Homologous genes for an alkane hydroxylase system (alk system) involved in liquid n-alkane oxidation were also cloned from the genomic DNA of Mycobacterium sp. TY-6. The alk gene cluster was transcribed in response to liquid n-alkanes (C-11-C-15). These results indicate that Mycobacterium sp. TY-6 has two distinct gene clusters for multicomponent monooxygenases involved in alkane oxidation. Whole-cell reactions revealed that propane is oxidized to I-propanol through terminal oxidation in Mycobacterium sp. TY-6 and that propane is oxidized to 1-propanol and 2-propanol through both terminal and subterminal oxidations in Pseudonocardia sp. TY-7. This study reveals the diversity of propane metabolism present in microorganisms.
- リンク情報
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- DOI
- https://doi.org/10.1263/jbb.102.184
- CiNii Articles
- http://ci.nii.ac.jp/naid/110004809590
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17046531
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000242001500007&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1263/jbb.102.184
- ISSN : 1389-1723
- CiNii Articles ID : 110004809590
- PubMed ID : 17046531
- Web of Science ID : WOS:000242001500007