2013年9月
Nicotinamide is a specific inhibitor of dark-operative protochlorophyllide oxidoreductase, a nitrogenase-like enzyme, from Rhodobacter capsulatus
FEBS LETTERS
- ,
- ,
- ,
- 巻
- 587
- 号
- 18
- 開始ページ
- 3142
- 終了ページ
- 3147
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.febslet.2013.07.054
- 出版者・発行元
- WILEY
Dark-operative protochlorophyllide oxidoreductase (DPOR) is a nitrogenase-like enzyme consisting of two components, L-protein as a reductase component and NB-protein as a catalytic component. Elucidation of the crystal structures of NB-protein (Muraki et al., Nature 2010, 465: 110-114) has enabled us to study its reaction mechanism in combination with biochemical analysis. Here we demonstrate that nicotinamide (NA) inhibits DPOR activity by blocking the electron transfer from L-protein to NB-protein. A reaction scheme of DPOR, in which the binding of protochlorophyllide (Pchlide) to the NB-protein precedes the electron transfer from the L-protein, is proposed based on the NA effects. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.febslet.2013.07.054
- ISSN : 1873-3468
- ORCIDのPut Code : 49957805
- Web of Science ID : WOS:000324033700039