2017年1月
Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA
MOLECULAR MEDICINE REPORTS
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- 巻
- 15
- 号
- 1
- 開始ページ
- 396
- 終了ページ
- 402
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3892/mmr.2016.5973
- 出版者・発行元
- SPANDIDOS PUBL LTD
Gas plasma, produced by a short high-voltage pulse generated from a static induction thyristor power supply [1.5 kilo pulse/sec (kpps)], was demonstrated to inactivate Geobacillus stearothermophilus spores (decimal reduction time at 15 min, 2.48 min). Quantitative polymerase chain reaction and enzyme-linked immunosorbent assays further indicated that nitrogen gas plasma treatment for 15 min decreased the level of intact genomic DNA and increased the level of 8-hydroxy-2'-deoxyguanosine, a major product of DNA oxidation. Three potential inactivation factors were generated during operation of the gas plasma instrument: Heat, longwave ultraviolet-A and oxidative stress (production of hydrogen peroxide, nitrite and nitrate). Treatment of the spores with hydrogen peroxide (3x2(-4)%) effectively inactivated the bacteria, whereas heat treatment (100 degrees C), exposure to UV-A (75-142 mJ/cm(2)) and 4.92 mM peroxynitrite (center dot ONOO-), which is decomposed into nitrite and nitrate, did not. The results of the present study suggest the gas plasma treatment inactivates bacterial spores primarily by generating hydrogen peroxide, which contributes to the oxidation of the host genomic DNA.
- リンク情報
- ID情報
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- DOI : 10.3892/mmr.2016.5973
- ISSN : 1791-2997
- eISSN : 1791-3004
- Web of Science ID : WOS:000393018700053