2001年11月
Determination of free D-proline and D-leucine in the brains of mutant mice lacking D-amino acid oxidase activity
ANALYTICAL BIOCHEMISTRY
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- 巻
- 298
- 号
- 2
- 開始ページ
- 253
- 終了ページ
- 258
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1006/abio.2001.5382
- 出版者・発行元
- ACADEMIC PRESS INC
A new procedure to accurately measure a trace amount Of D-proline in biological samples has been developed. This D-amino acid was derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole and was determined by a column-switching HPLC system, a combination of a micro-ODS column and a chiral column. The detection limit for D-proline spiked in a mouse cerebrum sample is 1 fmol (injection amount, S/N = 3). Within-day precision and day-to-day precision obtained for spiked D-proline (10 fmol) are 2.14 and 5.35% (RSD), respectively. Using the new method, the amount of free D-proline in eight brain regions and sera of mutant ddY/DAO(-) mice, lacking D-amino acid oxidase activity, and control ddY/DAO(+) mice was determined. The amount of free D-leucine was also investigated. The amount and distribution Of D-proline in the brains of ddY/DAO(+) mice and ddY/DAO- mice are almost the same, and relatively high amounts Of D-proline have been observed in the pituitary gland and in the pineal gland. On the other hand, the amount Of D-leucine is different between the two strains. In the brains of ddY/DAO(+) mice, a relatively high amount Of D-leucine has been observed in the pineal gland compared with other regions. In the brains of ddY/DAO(-) mice, D-leucine amounts are approximately 10 times higher than those obtained in ddY/DAO(+) mice and regional difference has not been observed, while the amounts of L-proline and L-leucine are not significantly different between the two strains. In the serum, the amounts of both free D-proline and D-leucine are significantly higher in the ddY/DAO- mice than those obtained in ddY/DAO(+) mice. (C) 2001 Academic Press.
- リンク情報
- ID情報
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- DOI : 10.1006/abio.2001.5382
- ISSN : 0003-2697
- Web of Science ID : WOS:000172451600013