2008年5月
Modulation of D-serine levels in brains of mice lacking PICK1
BIOLOGICAL PSYCHIATRY
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- 巻
- 63
- 号
- 10
- 開始ページ
- 997
- 終了ページ
- 1000
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.biopsych.2007.09.025
- 出版者・発行元
- ELSEVIER SCIENCE INC
Background: D-serine is an endogenous coagonist of the N-methyl-D-aspartate subtype glutamate receptor. Genetic association studies have implicated genes coding for enzymes associated with D-serine metabolism in schizophrenia and bipolar disorder.
Methods: Protein expression of serine racemase (SR) and its binding partner, protein interacting with C-kinase (PICK1), were examined by Western blotting in brains from wildtype and PICK1 knockout mice. Levels of D-serine in wildtype and PICK1 mice were also examined by an established high-pressure liquid chromatography protocol.
Results: Expression of SR and PICK1 proteins was developmentally regulated. Although no change was observed in the level of SR protein, levels of D-serine were selectively decreased in the forebrain of neonatal PICK1 knockout mice, compared with those in wildtype mice.
Conclusions: PICK1 may be involved in the regulation of brain D-serine levels and SR in a spatially and temporally specific manner.
Methods: Protein expression of serine racemase (SR) and its binding partner, protein interacting with C-kinase (PICK1), were examined by Western blotting in brains from wildtype and PICK1 knockout mice. Levels of D-serine in wildtype and PICK1 mice were also examined by an established high-pressure liquid chromatography protocol.
Results: Expression of SR and PICK1 proteins was developmentally regulated. Although no change was observed in the level of SR protein, levels of D-serine were selectively decreased in the forebrain of neonatal PICK1 knockout mice, compared with those in wildtype mice.
Conclusions: PICK1 may be involved in the regulation of brain D-serine levels and SR in a spatially and temporally specific manner.
- リンク情報
- ID情報
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- DOI : 10.1016/j.biopsych.2007.09.025
- ISSN : 0006-3223
- PubMed ID : 18191108
- Web of Science ID : WOS:000255604000012