1991年
GENERATION AND CHARACTERIZATION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 MUTANTS
ARCHIVES OF VIROLOGY
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- 巻
- 117
- 号
- 1-2
- 開始ページ
- 45
- 終了ページ
- 58
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/BF01310491
- 出版者・発行元
- SPRINGER-VERLAG WIEN
Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17,p24, and p15 regions), pol(protease, reverse transcriptase, and endonuclease domains), env (gp120 region), tat, or rev were infectious, whereas vif, vpr, vpu, some of env (gp41) and nef mutants could grow in human CD4+ cells to various degrees. Of the non-infectious mutants, only endonuclease (pol) and gp41 mutants exhibited normal phenotypes with respect to the production of functional reverse transcriptase, the expression of gag, pol, and env proteins, and the generation of progeny virions, when examined in transient assays. All infectious mutants killed the CD4+ cells with the exception of a mutant carrying a defect in the vif gene.
- リンク情報
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- DOI
- https://doi.org/10.1007/BF01310491
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902042162978507
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/1706590
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1991FD14600004&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1007/BF01310491
- ISSN : 0304-8608
- J-Global ID : 200902042162978507
- PubMed ID : 1706590
- Web of Science ID : WOS:A1991FD14600004