2017年4月
Monitoring ATP dynamics in electrically active white matter tracts
ELIFE
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- 巻
- 6
- 号
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.7554/eLife.24241
- 出版者・発行元
- ELIFE SCIENCES PUBLICATIONS LTD
In several neurodegenerative diseases and myelin disorders, the degeneration profiles of myelinated axons are compatible with underlying energy deficits. However, it is presently impossible to measure selectively axonal ATP levels in the electrically active nervous system. We combined transgenic expression of an ATP-sensor in neurons of mice with confocal FRET imaging and electrophysiological recordings of acutely isolated optic nerves. This allowed us to monitor dynamic changes and activity-dependent axonal ATP homeostasis at the cellular level and in real time. We find that changes in ATP levels correlate well with compound action potentials. However, this correlation is disrupted when metabolism of lactate is inhibited, suggesting that axonal glycolysis products are not sufficient to maintain mitochondrial energy metabolism of electrically active axons. The combined monitoring of cellular ATP and electrical activity is a novel tool to study neuronal and glial energy metabolism in normal physiology and in models of neurodegenerative disorders.
- リンク情報
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- DOI
- https://doi.org/10.7554/eLife.24241
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28414271
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415357
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000400576500001&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/28414271
- URL
- http://orcid.org/0000-0002-1896-0443
- ID情報
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- DOI : 10.7554/eLife.24241
- ISSN : 2050-084X
- ORCIDのPut Code : 33757927
- PubMed ID : 28414271
- PubMed Central 記事ID : PMC5415357
- Web of Science ID : WOS:000400576500001