2014年2月
Identification of truncated forms of U1 snRNA reveals a novel RNA degradation pathway during snRNP biogenesis
NUCLEIC ACIDS RESEARCH
- 巻
- 42
- 号
- 4
- 開始ページ
- 2708
- 終了ページ
- 2724
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/gkt1271
- 出版者・発行元
- OXFORD UNIV PRESS
The U1 small nuclear ribonucleoprotein (snRNP) plays pivotal roles in pre-mRNA splicing and in regulating mRNA length and isoform expression; however, the mechanism of U1 snRNA quality control remains undetermined. Here, we describe a novel surveillance pathway for U1 snRNP biogenesis. Mass spectrometry-based RNA analysis showed that a small population of SMN complexes contains truncated forms of U1 snRNA (U1-tfs) lacking the Sm-binding site and stem loop 4 but containing a 7-monomethylguanosine 5' cap and a methylated first adenosine base. U1-tfs form a unique SMN complex, are shunted to processing bodies and have a turnover rate faster than that of mature U1 snRNA. U1-tfs are formed partly from the transcripts of U1 genes and partly from those lacking the 3' box elements or having defective SL4 coding regions. We propose that U1 snRNP biogenesis is under strict quality control: U1 transcripts are surveyed at the 3'-terminal region and U1-tfs are diverted from the normal U1 snRNP biogenesis pathway.
- リンク情報
-
- DOI
- https://doi.org/10.1093/nar/gkt1271
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24311566
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000332381000058&DestApp=WOS_CPL
- URL
- http://orcid.org/0000-0003-3793-6219
- ID情報
-
- DOI : 10.1093/nar/gkt1271
- ISSN : 0305-1048
- eISSN : 1362-4962
- ORCIDのPut Code : 31588698
- PubMed ID : 24311566
- Web of Science ID : WOS:000332381000058