2017年
Development of heterotopic transplantation of the testis with the epididymis to evaluate an aspect of testicular immunology in rats.
PloS one
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- 巻
- 12
- 号
- 5
- 開始ページ
- e0177067
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0177067
Transplantation of testicular cells and tissues has been studied for the investigation of immunology of the testis, which is an immunologically privileged organ. However, reports of transplant of the testis at organ level have been extremely limited because of technical difficulties of the orthotopic testis transplantation (OTT) in experimental animals. In the present study, we developed a new and simple model of the heterotopic testis transplantation (HTT), which is donor testis transplantation into the cervical region of recipients, in a syngeneic model in rats [donor Lewis (LEW) graft to LEW recipient]. The duration of HTT was significantly shorter and success rate higher than that of OTT. To histologically evaluate HTT, the local immune responses were compared among the syngeneic model, an acute rejection allogeneic model [donor Augustus Copenhagen Irish (ACI) graft to LEW recipient] and a chronic rejection allogeneic model (donor F344 graft to LEW recipient) at postoperative day 3. We found that allogeneic ACI grafts resulted in mild and not severe orchitic lesions, whereas immune responses of allogeneic F344 grafts seemed intact and were not significantly different from those of syngeneic LEW grafts. These results suggest that our new operative procedure will be useful in future for the investigation of the testicular immunology.
- リンク情報
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- DOI
- https://doi.org/10.1371/journal.pone.0177067
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28475594
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5419600
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000400649500040&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1371/journal.pone.0177067
- ISSN : 1932-6203
- PubMed ID : 28475594
- PubMed Central 記事ID : PMC5419600
- Web of Science ID : WOS:000400649500040