As the boundary between glioblastoma and normal tissue is unclear, precise resection is required to reduce the risk of post-operative adverse event such as aphasia and to elongate residual life of the patient by resecting tumor region completely. Surgeons use various information such as preoperative and intraoperative image, surgical navigation, pathological images, and so on, to decide resection line. In conventional operating room, information is dispersed. In this study, video information integration system was implemented using dedicated hardware video mixer. In clinical evaluation, pre-set window layout was selected just by pushing a button, and neither delay nor noise was observed. In the future study, we will implement automatic layout changing system depending on the surgical scenario.