2018年
Synthesis and structural investigation of a series of mannose-containing oligosaccharides using mass spectrometry
Organic and Biomolecular Chemistry
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- 巻
- 16
- 号
- 2
- 開始ページ
- 228
- 終了ページ
- 238
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/c7ob02723k
- 出版者・発行元
- Royal Society of Chemistry
A series of compounds associated with naturally occurring and biologically relevant glycans consisting of α-mannosides were prepared and analyzed using collision-induced dissociation (CID), energy-resolved mass spectrometry (ERMS), and 1H nuclear magnetic resonance spectroscopy. The CID experiments of sodiated species of disaccharides and ERMS experiments revealed that the order of stability of mannosyl linkages was as follows: 6-linked >
4-linked ≧ 2-linked >
3-linked mannosyl residues. Analysis of linear trisaccharides revealed that the order observed in disaccharides could be applied to higher glycans. A branched trisaccharide showed a distinct dissociation pattern with two constituting disaccharide ions. The estimation of the content of this ion mixture was possible using the disaccharide spectra. The hydrolysis of mannose linkages at 3- and 6-positions in the branched trisaccharide revealed that the 3-linkage was cleaved twice as fast as the 6-linkage. It was observed that the solution-phase hydrolysis and gas-phase dissociation have similar energetics.
4-linked ≧ 2-linked >
3-linked mannosyl residues. Analysis of linear trisaccharides revealed that the order observed in disaccharides could be applied to higher glycans. A branched trisaccharide showed a distinct dissociation pattern with two constituting disaccharide ions. The estimation of the content of this ion mixture was possible using the disaccharide spectra. The hydrolysis of mannose linkages at 3- and 6-positions in the branched trisaccharide revealed that the 3-linkage was cleaved twice as fast as the 6-linkage. It was observed that the solution-phase hydrolysis and gas-phase dissociation have similar energetics.
- リンク情報
-
- DOI
- https://doi.org/10.1039/c7ob02723k
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/29234770
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85040196689&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85040196689&origin=inward
- URL
- http://orcid.org/0000-0003-4147-6868
- ID情報
-
- DOI : 10.1039/c7ob02723k
- ISSN : 1477-0520
- ORCIDのPut Code : 39585292
- PubMed ID : 29234770
- SCOPUS ID : 85040196689