A new method for determining the absolute detection efficiencies of a microchannel plate (MCP) detector is presented. This method uses a charge transfer reaction and can be applied to determine not only the detection efficiencies for ions but also those for neutral atoms. The measurements were carried out for Ar+ and neutral rare gas atoms (Ne, Ar, Kr and Xe) in the 0.5-4.8 keV energy range. The detection efficiencies for all species increase with increasing impact energy and approach the open area ratio of the MCP used (about 50%). The measured detection efficiencies were found to scale with the Lindhard, Scharff and Schiott formula for electronic stopping power at keV energies. © 2011 The Royal Swedish Academy of Sciences.