論文

査読有り
2016年

Estimation of methanogenesis by quantification of coenzyme F430 in marine sediments

GEOCHEMICAL JOURNAL
  • Masanori Kaneko
  • ,
  • Yoshinori Takano
  • ,
  • Nanako O. Ogawa
  • ,
  • Yuki Sato
  • ,
  • Naohiro Yoshida
  • ,
  • Naohiko Ohkouchi

50
6
開始ページ
453
終了ページ
460
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2343/geochemj.2.0410
出版者・発行元
GEOCHEMICAL SOC JAPAN

Coenzyme F430 is the hydrocorphinoid nickel complex which acts as active site in methyl-coenzyme M reductase (MCR). The MCR-F430 complex catalyzes the last step of methanogenesis: reduction of methyl-coenzyme M to methane. Since F430 is a common enzyme for mathanogens, it can be a function-specific biomarker to estimate biomass and activities of methanogens in environmental samples. A recently developed high sensitive method for coenzyme F430 analysis and a further purification step revealed F430 concentrations at 69 and 88 meters below seafloor (mbsf) off Shimokita Peninsula (529 and 31.3 fmol g-wet(-1)) and at 60 and 275 mbsf at Nankai Trough (31.4 and 26 fmol g-wet(-1)). Estimated methanogenic biomass and activities based on reported F430 content and activities in methanogens and prokaryotic cell weight, were ranging from 3.9 +/- 10(6) to 2.7 +/- 10(6) cells g-wet(-1) and from 1.7 to 11 mu mol d(-1) g-wet(-1).

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.2343/geochemj.2.0410
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000392504000004&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-85007352153&partnerID=MN8TOARS
URL
http://orcid.org/0000-0003-0787-4731

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