2009年10月
Identification of functional marker proteins in the mammalian growth cone
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- 巻
- 106
- 号
- 40
- 開始ページ
- 17211
- 終了ページ
- 17216
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.0904092106
- 出版者・発行元
- NATL ACAD SCIENCES
Identification of proteins in the mammalian growth cone has the potential to advance our understanding of this critical regulator of neuronal growth and formation of neural circuit; however, to date, only one growth cone marker protein, GAP-43, has been reported. Here, we successfully used a proteomic approach to identify 945 proteins present in developing rat forebrain growth cones, including highly abundant, membrane-associated and actin-associated proteins. Almost 100 of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 17 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus their identification presents a significant step forward, providing marker proteins and candidate neuronal growth-associated proteins.
- リンク情報
- ID情報
-
- DOI : 10.1073/pnas.0904092106
- ISSN : 0027-8424
- PubMed ID : 19805073
- Web of Science ID : WOS:000270537500063