2018年
Enzymatically cleavable traceless biotin tags for protein PEGylation and purification
Chemical Communications
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- 巻
- 54
- 号
- 15
- 開始ページ
- 1913
- 終了ページ
- 1916
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/c7cc05814d
- 出版者・発行元
- Royal Society of Chemistry
Here we report an example of a protein-PEG conjugate with a biotin tag cleavable by lipase-catalyzed hydrolysis. Very mild cleavage conditions, heterogeneous, easily separable catalysts, and traceless design make this method attractive for the preparation and purification of PEGylated proteins.
- ID情報
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- DOI : 10.1039/c7cc05814d
- ISSN : 1364-548X
- ISSN : 1359-7345
- PubMed ID : 29393938
- SCOPUS ID : 85042191993