1996年3月
Transfection of wild-type TP53 induces differentiation in human gingival carcinoma cells
EUROPEAN JOURNAL OF CANCER
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 32A
- 号
- 3
- 開始ページ
- 533
- 終了ページ
- 539
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/0959-8049(95)00611-7
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
We investigated the effects of transfection of wild-type TP53 on the growth properties of a human gingival carcinoma cell line, KOSC-3, in which the TP53 gene is mutated at codon 248 and overexpressed. The wild-type TP53 expression plasmid, pCDM8-p53/neo and the control plasmid, pCDM8/neo, were each stably transfected into KOSC-3 cells by using the calcium phosphate method. The number of G418-resistant colonies hom wild-type TP53-transfected cells was approximately half that from plasmid controls. Exogenous wild-type TP53 transcripts were identified in four of the 20 G418-resistant clones analysed by reverse transcription PCR. Although the growth rates of the wildtype TP53+ clones did not drastically change curing log phase, their saturation density was significantly reduced. The wild-type TP53+ cells were morphologically flat and enlarged when cultured in vitro, and were less able to form colonies in soft agar. In nude mice, the wild-type TP53+ clones formed subcutaneous tumours with conspicuous keratinisation and notable cell death that was not manifested in the parental and plasmid control cells. These findings indicate that the wild-type TP53 gene, even when it coexists with a mutated form, may function as a growth suppressor and differentiation inducer under restricted conditions in gingival squamous cell carcinoma.
- リンク情報
- ID情報
-
- DOI : 10.1016/0959-8049(95)00611-7
- ISSN : 0959-8049
- PubMed ID : 8814703
- Web of Science ID : WOS:A1996UC27500030