2008年12月
The Presence of Foxp3 Expressing T Cells Within Grafts of Tolerant Human Liver Transplant Recipients
TRANSPLANTATION
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- 巻
- 86
- 号
- 12
- 開始ページ
- 1837
- 終了ページ
- 1843
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1097/TP.0b013e31818febc4
- 出版者・発行元
- LIPPINCOTT WILLIAMS & WILKINS
Background. Some experimental transplant-tolerance models have shown that the presence of regulatory T cells within grafts is important for the development of tolerance (Tol).
Methods. To determine if the presence of regulatory T cells correlates with graft acceptance in living-donor liver-transplantation tolerance, the expression of Foxp3 mRNA and the presence of CD4(+), CD8(+), and Foxp3(+) cells were quantified in biopsies from tolerant recipients by real-time polymerase chain reaction and by immunohistochemistry and immunofluorescent staining (Gr-Tol). The results were compared with biopsies from the recipients oil maintenance immunosuppression (Gr-IS), grafts removed because of chronic rejection (Gr-CR), or normal liver (Gr-NL).
Results. The expression of Foxp3 mRNA in Gr-Tol was higher than that in Gr-IS (P=0.07) and Gr-NL (P<0.0001), but equivalent to that in Gr-CR. In Gr-Tol, Foxp3(+) cells were detectable within the clustered CD4(+) and CD8(+) cells in the portal areas. Ninety-two percent of those Foxp3(+) cells were CD4(+), whereas 8% were CD8(+). The number of Foxp3(+) cells was significantly increased in Gr-Tol, compared with that in Gr-IS (P<0.05), although the number of CD4(+) or CD8(+) cells did not differ between the two. Foxp3(+) cells were hardly detectable in Gr-CR or -NL.
Conclusions. This is the first report showing that CD4(+) Foxp3(+) cells are present within grafts in a subset of tolerant patients after human liver transplantation. A prospective study is needed to elucidate whether the assessment of intragraft expression of Foxp3 protein, but not Foxp3 mRNA, can aid the identification of living-donor liver-transplantation recipients who can successfully withdraw IS.
Methods. To determine if the presence of regulatory T cells correlates with graft acceptance in living-donor liver-transplantation tolerance, the expression of Foxp3 mRNA and the presence of CD4(+), CD8(+), and Foxp3(+) cells were quantified in biopsies from tolerant recipients by real-time polymerase chain reaction and by immunohistochemistry and immunofluorescent staining (Gr-Tol). The results were compared with biopsies from the recipients oil maintenance immunosuppression (Gr-IS), grafts removed because of chronic rejection (Gr-CR), or normal liver (Gr-NL).
Results. The expression of Foxp3 mRNA in Gr-Tol was higher than that in Gr-IS (P=0.07) and Gr-NL (P<0.0001), but equivalent to that in Gr-CR. In Gr-Tol, Foxp3(+) cells were detectable within the clustered CD4(+) and CD8(+) cells in the portal areas. Ninety-two percent of those Foxp3(+) cells were CD4(+), whereas 8% were CD8(+). The number of Foxp3(+) cells was significantly increased in Gr-Tol, compared with that in Gr-IS (P<0.05), although the number of CD4(+) or CD8(+) cells did not differ between the two. Foxp3(+) cells were hardly detectable in Gr-CR or -NL.
Conclusions. This is the first report showing that CD4(+) Foxp3(+) cells are present within grafts in a subset of tolerant patients after human liver transplantation. A prospective study is needed to elucidate whether the assessment of intragraft expression of Foxp3 protein, but not Foxp3 mRNA, can aid the identification of living-donor liver-transplantation recipients who can successfully withdraw IS.
- リンク情報
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- DOI
- https://doi.org/10.1097/TP.0b013e31818febc4
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201502876216321571
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19104431
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000262053500037&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1097/TP.0b013e31818febc4
- ISSN : 0041-1337
- J-Global ID : 201502876216321571
- PubMed ID : 19104431
- Web of Science ID : WOS:000262053500037