2013年8月
Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase
NATURE COMMUNICATIONS
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- 巻
- 4
- 号
- 開始ページ
- 2191
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/ncomms3191
- 出版者・発行元
- NATURE PUBLISHING GROUP
Leguminous plants establish a symbiosis with rhizobia to enable nitrogen fixation in root nodules under the control of the presumed root-to-shoot-to-root negative feedback called autoregulation of nodulation. In Lotus japonicus, autoregulation is mediated by CLE-RS genes that are specifically expressed in the root, and the receptor kinase HAR1 that functions in the shoot. However, the mature functional structures of CLE-RS gene products and the molecular nature of CLE-RS/HAR1 signalling governed by these spatially distant components remain elusive. Here we show that CLE-RS2 is a post-translationally arabinosylated glycopeptide derived from the CLE domain. Chemically synthesized CLE-RS glycopeptides cause significant suppression of nodulation and directly bind to HAR1 in an arabinose-chain and sequence-dependent manner. In addition, CLE-RS2 glycopeptide specifically produced in the root is found in xylem sap collected from the shoot. We propose that CLE-RS glycopeptides are the long sought mobile signals responsible for the initial step of autoregulation of nodulation.
- リンク情報
- ID情報
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- DOI : 10.1038/ncomms3191
- ISSN : 2041-1723
- PubMed ID : 23934307
- Web of Science ID : WOS:000323749500001