論文

査読有り
2020年5月29日

Characterization of colibactin-producing Escherichia coli isolated from Japanese patients with colorectal cancer.

Japanese journal of infectious diseases
  • Yuko Yoshikawa
  • ,
  • Yuta Tsunematsu
  • ,
  • Nobuo Matsuzaki
  • ,
  • Yuichiro Hirayama
  • ,
  • Fumi Higashiguchi
  • ,
  • Michio Sato
  • ,
  • Yuji Iwashita
  • ,
  • Noriyuki Miyoshi
  • ,
  • Michihiro Mutoh
  • ,
  • Hideki Ishikawa
  • ,
  • Haruhiko Sugimura
  • ,
  • Keiji Wakabayashi
  • ,
  • Kenji Watanabe

73
6
開始ページ
437
終了ページ
442
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.7883/yoken.JJID.2020.066

We investigated the relationship between colibactin-producing (clb+) Escherichia coli and colorectal adenocarcinoma. In total, 729 E. coli colonies were isolated from tumor and surrounding non-tumor regions in resected specimens from 34 Japanese patients; 450 colonies were from tumor regions and 279 from non-tumor regions. clb+ bacteria were found in tumor regions of 11 patients (11/34, 32.4%) and in non-tumor regions of seven of the 11 (7/34, 20.6%). The prevalence of clb+ isolates was 72.7% (327/450) and 44.1% (123/279) in tumor and non-tumor regions, respectively. All the recovered clb+ isolates belonged to the phylogenetic group B2 and were the most predominant type in tumor regions. Hemolytic (α-hemolysin-positive, hlyA+) and non-hemolytic (α-hemolysin-negative, hlyA-) clb+ isolates were obtained from patient #19; however, the prevalence of hlyA+ clb+ isolates was significantly higher in tumor regions (35/43, 81.4%) than in non-tumor regions (3/19, 15.8%). Moreover, a significantly higher production of N-myristoyl-D-asparagine, a byproduct of colibactin biosynthesis, was observed in hlyA+ clb+ isolates than in hlyA- clb+ isolates. Our results suggest that hlyA+ clb+ E. coli may have a selective advantage in colorectal colonization, consequently playing a role in carcinogenesis. The presence of hlyA+ clb+ bacteria in healthy individuals is a risk marker of colorectal cancer.

リンク情報
DOI
https://doi.org/10.7883/yoken.JJID.2020.066
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32475872
ID情報
  • DOI : 10.7883/yoken.JJID.2020.066
  • PubMed ID : 32475872

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