Burkholderia sp. NK8 can utilize 3-chlorobenzoate (3CB) as a sole source of carbon because it has a megaplasmid (pNK8) that carries the gene cluster (tfdT-CDEF) encoding chlorocatechol-degrading enzymes. The expression of tfdT-CDEF is induced by 3CB. In this study, we found that NK8 cells were attracted to 3CB and its degradation products, 3- and 4-chlorocatechol, and beta-ketoadipate. Capillary assays revealed that a pNK8-eliminated strain (NK82) was defective in chemotaxis toward beta-ketoadipate. The introduction of a plasmid carrying a putative outer membrane porin gene, which we name omp(NK8), into strain NK82 restored chemotaxis toward beta-ketoadipate. RT-PCR analyses demonstrated that the transcription of the omp(NK8) gene was enhanced in the presence of 3CB.