Chikashi Toyoshima

Profile Information

Affiliation: Professor,Professor, Institute of Molecular and Cellular Biosciences, Department of Structural Biology, Laboratory of Supramolecular Structure, The University of Tokyo

Degree: 理学博士(東京大学)

J-GLOBAL ID: 200901037323556511
researchmap Member ID: 0000110047

External link: http://www.iam.u-tokyo.ac.jp/StrBiol/

Research Interests

Research Areas

Life sciences / Biophysics /

Research History

Apr, 2020 - Present

東京大学定量生命科学研究所特任教授、東京大学特別教授室特別教授
Apr, 2018 - Mar, 2020

改組により東京大学定量生命科学研究所教授
1994

Institute of Molecular and Cellular Biosciences, The University of Tokyo
1990

School of Science, Tokyo Institute of Technology
1989

理化学研究所･国際フロンティア研究員
1988

英国MRC分子生物学研究所研究員
1986

米国スタンフォード大学細胞生物学教室博士研究員
1984

Faculty of Science, The University of Tokyo
現在に至る

Education

- 1983

The University of Tokyo
- 1978

Department of Physics, Faculty of Science, The University of Tokyo

Awards

Jun, 2018

Imperial Prize and Japan Academy Prize, Elucidation of the Molecular Mechanism of the ATP-driven Ion-transport across the Membranes, The Japan Academy

TOYOSHIMA Chikashi
2016

2016年度武田医学賞, 武田科学振興財団

豊島近
2016

平成27年度上原賞, 上原記念生命科学財団

豊島近
2016

Gregori Aminoff Prize, スウェーデン王立科学アカデミー

豊島近
2015

紫綬褒章

豊島近
2011

第11回山崎貞一賞, 材料科学技術振興財団

豊島近
2010

2009年度朝日賞, 朝日新聞社

豊島近
2008

Michael Berridge賞, 欧州カルシウム学会

豊島近
2005

米国科学アカデミー外国人会員
2005

Foreign Associate of the National Academy of Sciences,USA

Papers

Crystal structures of Na+ ,K+ -ATPase reveal the mechanism that converts the K+ -bound form to Na+ -bound form and opens and closes the cytoplasmic gate.

Ryuta Kanai, Bente Vilsen, Flemming Cornelius, Chikashi Toyoshima

FEBS letters, Jun 26, 2023
Cryo-electron microscopy of Na+ ,K+ -ATPase reveals how the extracellular gate locks in the E2·2K+ state.

Ryuta Kanai, Flemming Cornelius, Bente Vilsen, Chikashi Toyoshima

FEBS letters, Jun 23, 2022
Cryoelectron microscopy of Na+,K+-ATPase in the two E2P states with and without cardiotonic steroids.

Ryuta Kanai, Flemming Cornelius, Bente Vilsen, Chikashi Toyoshima

Proceedings of the National Academy of Sciences of the United States of America, 119(15) e2123226119, Apr 12, 2022
Binding of cardiotonic steroids to Na+,K+-ATPase in the E2P state.

Ryuta Kanai, Flemming Cornelius, Haruo Ogawa, Kanna Motoyama, Bente Vilsen, Chikashi Toyoshima

Proceedings of the National Academy of Sciences of the United States of America, 118(1), Jan 7, 2021 Peer-reviewed
What ATP binding does to the Ca2+ pump and how nonproductive phosphoryl transfer is prevented in the absence of Ca2.

Yoshiki Kabashima, Haruo Ogawa, Rie Nakajima, Chikashi Toyoshima

Proceedings of the National Academy of Sciences of the United States of America, 117(31) 18448-18458, Aug 4, 2020 Peer-reviewed
Mechanism of the E2 to E1 transition in Ca2+ pump revealed by crystal structures of gating residue mutants

Tsunekawa Naoki, Ogawa Haruo, Tsueda Junko, Akiba Toshihiko, Toyoshima Chikashi

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115(50) 12722-12727, Dec 11, 2018 Peer-reviewed
Suppressor mutation analysis combined with 3d modeling explains cohesin’s capacity to hold and release DNA

Xingya Xu, Ryuta Kanai, Norihiko Nakazawa, Li Wang, Chikashi Toyoshima, Mitsuhiro Yanagida

Proceedings of the National Academy of Sciences of the United States of America, 115(21) E4833-E4842, May 22, 2018 Peer-reviewed
Distinct pH dependencies of Na+/K+ selectivity at the two faces of Na,K-ATPase

Flemming Cornelius, Naoki Tsunekawa, Chikashi Toyoshima

Journal of Biological Chemistry, 293(6) 2195-2205, 2018 Peer-reviewed
Protein-phospholipid interplay revealed with crystals of a calcium pump

Yoshiyuki Norimatsu, Kazuya Hasegawa, Nobutaka Shimizu, Chikashi Toyoshima

NATURE, 545(7653) 193-+, May, 2017 Peer-reviewed
Mn2+ transport by Ca2+-ATPase of sarcoplasmic reticulum (vol 590, pg 2086, 2016)

Shin-Ichiro Yonekura, Chikashi Toyoshima

FEBS LETTERS, 590(24) 4650-4650, Dec, 2016 Peer-reviewed
Mn2+ transport by Ca2+-ATPase of sarcoplasmic reticulum

Shin-Ichiro Yonekura, Chikashi Toyoshima

FEBS LETTERS, 590(14) 2086-2095, Jul, 2016 Peer-reviewed
Functional analysis of SERCA1b, a highly expressed SERCA1 variant in myotonic dystrophy type 1 muscle.

Zhao Y, Ogawa H, Yonekura S, Mitsuhashi H, Mitsuhashi S, Nishino I, Toyoshima C, Ishiura S

Biochimica et biophysica acta, 1852(10 Pt A) 2042-2047, Oct, 2015 Peer-reviewed
Functional analysis of SERCA1b, a highly expressed SERCA1 variant in myotonic dystrophy type 1 muscle

Yimeng Zhao, Haruo Ogawa, Shin-Ichiro Yonekura, Hiroaki Mitsuhashi, Satomi Mitsuhashi, Ichizo Nishino, Chikashi Toyoshima, Shoichi Ishiura

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1852(10) 2042-2047, Oct, 2015 Peer-reviewed
Sequential substitution of K+ bound to Na+, K+-ATPase visualized by X-ray crystallography

Haruo Ogawa, Flemming Cornelius, Ayami Hirata, Chikashi Toyoshima

NATURE COMMUNICATIONS, 6 8004, Aug, 2015 Peer-reviewed
Biselyngbyasides, cytotoxic marine macrolides, are novel and potent inhibitors of the Ca2+ pumps with a unique mode of binding

Maho Morita, Haruo Ogawa, Osamu Ohno, Takao Yamori, Kiyotake Suenaga, Chikashi Toyoshima

FEBS LETTERS, 589(13) 1406-1411, Jun, 2015 Peer-reviewed
Electron crystallography of ultrathin 3D protein crystals: Atomic model with charges

Koji Yonekura, Kazuyuki Kato, Mitsuo Ogasawara, Masahiro Tomita, Chikashi Toyoshima

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(11) 3368-3373, Mar, 2015 Peer-reviewed
Stimulation, Inhibition, or Stabilization of Na,K-ATPase Caused by Specific Lipid Interactions at Distinct Sites

Michael Habeck, Haim Haviv, Adriana Katz, Einat Kapri-Pardes, Sophie Ayciriex, Andrej Shevchenko, Haruo Ogawa, Chikashi Toyoshima, Steven J. D. Karlish

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(8) 4829-4842, Feb, 2015 Peer-reviewed
Design, synthesis and structure-activity relationship studies of novel sirtuin 2 (SIRT2) inhibitors with a benzamide skeleton

Taki Sakai, Yotaro Matsumoto, Minoru Ishikawa, Kazuyuki Sugita, Yuichi Hashimoto, Nobuhiko Wakai, Akio Kitao, Era Morishita, Chikashi Toyoshima, Tomoatsu Hayashi …

BIOORGANIC & MEDICINAL CHEMISTRY, 23(2) 328-339, Jan, 2015 Peer-reviewed
ATPase-dependent auto-phosphorylation of the open condensin hinge diminishes DNA binding

Yuko Akai, Ryuta Kanai, Norihiko Nakazawa, Masahiro Ebe, Chikashi Toyoshima, Mitsuhiro Yanagida

OPEN BIOLOGY, 4(12), Dec, 2014 Peer-reviewed
5-Hydroxymethylcytosine Plays a Critical Role in Glioblastomagenesis by Recruiting the CHTOP-Methylosome Complex

Hiroki Takai, Koji Masuda, Tomohiro Sato, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki, Ryo Koyama-Nasu, Yukiko Nasu-Nishimura, Yuki Katou, Haruo Ogawa …

CELL REPORTS, 9(1) 48-60, Oct, 2014 Peer-reviewed

Misc.

122

Crystal structure analysis of P-type ATPase

TOYOSHIMA Chikashi

23(4) 334-342, Nov, 2018 Peer-reviewed
X線コントラスト変調法によって明らかになった膜蛋白質と燐脂質の相互作用のダイナミクス

豊島近, 乗松良行

放射光, 31(3) 212-218, May, 2018 Invited
はじめて解像された膜タンパク質とリン脂質の相互作用のダイナミクス

乗松良行, 豊島近

実験医学, 35(6) 2741-2744, Oct, 2017
Large production of mammalian membrane proteins toward determination of high-resolution structure

H. Ogawa, C. Toyoshima

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 46 S169-S169, Jul, 2017
Large Scale Production of Mammalian Membrane Proteins Toward Determination of High-Resolution Structure

Haruo Ogawa, Chikashi Toyoshima

BIOPHYSICAL JOURNAL, 112(3) 188A-188A, Feb, 2017
Kinetics by X-Ray Crystallography: Sequential Substitution of K+ Bound To Na+, K+-ATPase

Haruo Ogawa, Flemming Cornelius, Ayami Hirata, Chikashi Toyoshima

BIOPHYSICAL JOURNAL, 110(3) 629A-629A, Feb, 2016
X-Ray Crystallographic Study of Na,K-ATPase in Complex with Cardiotonic Steroids

Haruo Ogawa, Kanna Motoyama, Flemming Cornelius, Bente Vilsen, Chikashi Toyoshima

BIOPHYSICAL JOURNAL, 108(2) 197A-197A, Jan, 2015
Structural Biology of Ion Pumps (P-type ATPases)

TOYOSHIMA Chikashi

24(1) 2-9, Jan 30, 2011
STRUCTURAL ASPECTS OF CATION PUMPING BY P-TYPE ATPASES

Chikashi Toyoshima, Takehiro Shinoda, Haruo Ogawa, Flemming Cornelius

JOURNAL OF PHYSIOLOGICAL SCIENCES, 59 95-95, 2009
Conformational fluctuations of the Ca2+-ATPase in the native membrane environment - Effects of pH, temperature, catalytic substrates, and thapsigargin

Giuseppe Inesi, David Lewis, Chikashi Toyoshima, Ayami Hirata, Leopoldo de Meis

JOURNAL OF BIOLOGICAL CHEMISTRY, 283(2) 1189-1196, Jan, 2008
How processing of aspartylphosphate is coupled to lumenal gating of the ion pathway in the calcium pump

Chikashi Toyoshima, Yoshiyuki Norimatsu, Shiho Iwasawa, Takeo Tsuda, Haruo Ogawa

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104(50) 19831-19836, Dec, 2007
Structure determination of tubular crystals of membrane proteins. IV. Distortion correction and its combined application with real-space averaging and solvent flattening

Koji Yonekura, Chikashi Toyoshima

ULTRAMICROSCOPY, 107(12) 1141-1158, Nov, 2007
Domain organization and movements in heavy metal ion pumps - Papain digestion of CopA, a Cu+-transporting ATPase

Yuta Hatori, Eiji Majima, Takeo Tsuda, Chikashi Toyoshima

JOURNAL OF BIOLOGICAL CHEMISTRY, 282(35) 25213-25221, Aug, 2007
Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors

Mihoko Takahashi, Youhei Kondou, Chikashi Toyoshima

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104(14) 5800-5805, Apr, 2007
Structural aspects of ion pumping by Ca2+-ATPase of sarcoplasmic reticulum

Chikashi Toyoshima

New Comprehensive Biochemistry, 41 219-228, 2007
Functional consequences of site directed mutations at the distal end of the calcium binding cavity in the sarcoplasmic reticulum ATPase (SERCA).

Cheng Xu, Giuseppe -. Inesi, Chikashi Toyoshima

BIOPHYSICAL JOURNAL, 146A-146A, Jan, 2007
Concerted conformational effects of Ca2+ and ATP are required for activation of sequential reactions in the Ca2+ ATPase (SERCA) catalytic cycle

Giuseppe Inesi, David Lewis, Hailun Ma, Anand Prasad

BIOCHEMISTRY, 45(46) 13769-13778, Nov, 2006
Structural changes in the cytoplasmic domain of phospholamban by phosphorylation at Ser16: A molecular dynamics study

Yuji Sugita, Naoyuki Miyashita, Takao Yoda, Mitsunori Ikeguchi, Chikashi Toyoshima

Biochemistry, 45(39) 11752-11761, Oct 3, 2006
Effects of inhibitors on luminal opening of Ca2+ binding sites in an E2P-like complex of sarcoplasmic reticulum Ca2+-ATPase with Be2+-fluoride

M Picard, C Toyoshima, P Champeil

JOURNAL OF BIOLOGICAL CHEMISTRY, 281(6) 3360-3369, Feb, 2006
Structural role of countertransport revealed in Ca2+ pump crystal structure in the absence of Ca2+

K Obara, N Miyashita, C Xu, L Toyoshima, Y Sugita, G Inesi, C Toyoshima

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(41) 14489-14496, Oct, 2005

Books and Other Publications

Presentations

Structural Biology of Ion Pumps : From lipid bilayer to proton

Oct 30, 2019 Invited
P 型 ATPase によるイオン輸送とその制御機構

豊島近

日本薬学会第139年会, Mar 22, 2019 Invited
Role of phospholipids in ion pumping

TOYOSHIMA Chikashi

5th ShanghaiTech-SIAIS Bioforum, Nov 13, 2018 Invited
The current situation of crystal structure analysis of Ca pump

TOYOSHIMA Chikashi

Aug 9, 2018 Invited
Structural Biology of the Calcium Pump

TOYOSHIMA Chikashi

WCP2018 - 18th World Congress of Basic and Clinical Pharmacology, Jul 2, 2018 Invited
Structural Biology of Ion Pumps

TOYOSHIMA Chikashi

Post Symposium, Jun 8, 2018 Invited
Structural Biology of Ion Pumps

TOYOSHIMA Chikashi

The 2nd International Symposium on Chemical Communication, May 28, 2018 Invited
Sructural biology of P-type ion translocating ATPases: towards complete understanding of the mechanism.

TOYOSHIMA Chikashi

Aminoff Prize Symposium, Mar 30, 2016 Invited
イオンポンプ蛋白質の構造生物学とSPring-8

SPring-8シンポジウム2015, 2015 Invited
How Na+,K+ -ATPase select andpumps sodium across the membrane, Visualization of lipid bilayers in the crystals of calcium pump

TOYOSHIMA Chikashi

2nd NTU Membrane Protein Weeks, 2015 Invited
New crystal structures of Ca2+-ATPase(SERCA) and Na+,K+ -ATPases

TOYOSHIMA Chikashi

Barcelona BioMed Conference, 2014 Invited
New crystal structures of Ca2+-and Na+ ,K+ -ATPases

TOYOSHIMA Chikashi

ASBMB Special Symposia Series Na, K-ATPase and Related Transport ATPases, 2014 Invited
Structural Understanding of ion Pumping by P-type ATPases.

TOYOSHIMA Chikashi

Benzon Symposium No.59, 2013 Invited
イオンポンプの構造生物学：全ての反射点の利用

第13回日本蛋白質科学会年会, 2013 Invited
Structural study on the calcium ATPase: ion pumping mechanism and its regulation

TOYOSHIMA Chikashi

The 1st NRPB International Symposium and Workshop for Membrane Proteins: From Structure to Drug Discovery, 2012 Invited
イオンポンプ作動機構の構造的理解

学術振興会169委員会第37回研究会, 2011 Invited
Do lipid bilayers follow movements of transmembrane helices? If so, to what extent?

TOYOSHIMA Chikashi

The EMBO Meeting 2011, 2011 Invited
Visualization of lipid bilayers in protein crystals by contrast modulation.

TOYOSHIMA Chikashi

XXII Congress and General Assembly of the International Union of Crystallography, 2011 Invited
A Structural view on ion pumping by Ca2+-ATPase.

TOYOSHIMA Chikashi

2010FASEB Summer Research Conferences, Transport ATPase: From Molecules to Maladies., 2010 Invited
結晶構造から見たCa2+-ATPaseのイオン輸送・エネルギー変換機構

日本生体エネルギー研究会第35回討論会, 2009 Invited

Professional Memberships

日本生物物理学会

Research Projects

多機能化一本鎖抗体によるアミロイドβ凝集体細胞内スワイプ療法の開発

科学研究費助成事業, 挑戦的研究(開拓), 日本学術振興会, Jun, 2022 - Mar, 2025

星美奈子, 村松慎一, 笹原智也, 石井佳誉, 豊島近, 喜井勲
異常凝集体に対する体外診断法開発によるアルツハイマー病の早期診断と病態解明

科学研究費助成事業, 基盤研究(B), 日本学術振興会, Apr, 2020 - Mar, 2023

星美奈子, 村松慎一, 笹原智也, 豊島近, 久保厚子
Developement of technology for visualising lipid bilayers in crystals of membrane proteins

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Pioneering), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2022

Toyoshima Chikashi
Structural biology of active transport and its regulation by P-type ATPases

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2022
Visualization of Coulomb potential by electron crystallography

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

YONEKURA Koji, MAKI Saori, TOYOSHIMA Chikashi
P型イオンポンプによる能動輪送機構の構造的解明

科学研究費助成事業, 学術創成研究費, 日本学術振興会, 2002 - 2006

豊島近, 杉田有冶, 津田岳夫, 木寺詔紀, 鈴木裕
Structural Biology of Biological Machineries through X-ray Protein Crystallography Reinforced by Synchrotron Radiation

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, Japan Society for the Promotion of Science, 1998 - 2003

TANOKURA Masaru, TSUKIHARA Tomitake, MIURA Kinichiro, EBASHI Setsuro, TANAKA Isao, MIKI Kunio
Ca-ATPaseの電子線・X線結晶解析

科学研究費助成事業, 特定領域研究, 日本学術振興会, 1999 - 2002

豊島近, 津田岳夫, 小川治夫
Crystallographics study of active ion transport

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, 1999 - 2001

TOYOSHIMA Chikashi, OGAWA Haruo, NAKASAKO Masayoshi
Development of electron diffraction methods for protein crystallography

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 1998 - 1999

TOYOSHIMA Chikashi, SUZUKI Seiichi, OGASAWARA Mitsuo, NAKASAKO Masayoshi
Structural elucidation of active ion transport

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B)., Japan Society for the Promotion of Science, 1998 - 1999

TOYOSHIMA Chikashi, NAKASAKO Masayoshi
Crystallographic study of calcium ion pump of sarcoplasmic reticulum

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 1997 - 1998

TOYOSHIMA Chikashi
Development of the 4th generation calcium antagonists based on molecular revealing the binding sites in calcium channels

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, 1996 - 1998

NAKAYAMA Hitoshi, NABATA Hiroyuki, KUNIYASU Akihiko, KAWANO Seiko, ISHIZUKA Tadao
Structural Basis of Active Transport by Ion Pumps

Grants-in-Aid for Scientific Research, Grant-in-Aid for international Scientific Research, Japan Society for the Promotion of Science, 1996 - 1997

TOYOSHIMA Chikashi, OGAWA Haruo, STOKES David L
チャネルとトランスポータの構造・機能相関

科学研究費助成事業, 重点領域研究, 日本学術振興会, 1995 - 1997

岡田泰伸, 森吉弘毅, 豊島近, 鈴木誠, 佐々木成, 井本敬二
イオンチャンネル機能を実現する超分子構造の化学的,物理学的解析

科学研究費助成事業, 重点領域研究, 日本学術振興会, 1996 - 1996

中山仁, 豊島近, 國安明彦, 原田久美子, 中山守雄
Elucidation of the organization mechanism of dynamic higher-ordered structures in biological cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, 1995 - 1996

KOUYAMA Tsutomu, KASAI Michio, OOKI Kazuo, SAKO Yasushi, MIYAMOTO Shigeaki, ARISAKA Fumio
Conformation of the Muscarinic Acetylcholine Receptor

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, 1994 - 1996

HAGA Tatsuya, YOKOYAMA Shigeyuki, TOYOSHIMA Chikasi, FUJISAWA Tadami, SASAKI Hiromi, KAMEYAMA Kimihiko
イオンチャンネル機能を実現する超分子構造の化学的、物理学的解析

科学研究費助成事業, 重点領域研究, 日本学術振興会, 1995 - 1995

中山仁, 國安明彦, 豊島近, 原田久美子, 中山守雄
New Generation of Protein Crystallography

Grants-in-Aid for Scientific Research, Grant-in-Aid for Co-operative Research (A), Japan Society for the Promotion of Science, 1994 - 1995

MIKI Kunio, TOYOSHIMA Chikashi, TSUKIHARA Tomitake, FUKUYAMA Keiichi, HIGUCHI Yoshiki, TANAKA Isao

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Misc.

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