Aug, 2007
Cell-type specificity of the expression of os BOR1, a rice efflux boron transporter gene, is regulated in response to boron availability for efficient boron uptake and xylem loading
PLANT CELL
- ,
- ,
- ,
- ,
- ,
- Volume
- 19
- Number
- 8
- First page
- 2624
- Last page
- 2635
- Language
- English
- Publishing type
- DOI
- 10.1105/tpc.106.049015
- Publisher
- AMER SOC PLANT BIOLOGISTS
We describe a boron ( B) transporter, Os BOR1, in rice ( Oryza sativa). Os BOR1 is a plasma membrane-localized efflux transporter of B and is required for normal growth of rice plants under conditions of limited B supply (referred to as -B). Disruption of Os BOR1 reduced B uptake and xylem loading of B. The accumulation of Os BOR1 transcripts was higher in roots than that in shoots and was not affected by B deprivation; however, Os BOR1 was detected in the roots of wild-type plants under -B conditions, but not under normal conditions, suggesting regulation of protein accumulation in response to B nutrition. Interestingly, tissue specificity of Os BOR1 expression is affected by B treatment. Transgenic rice plants containing an Os BOR1 promoter-beta-glucuronidase (GUS) fusion construct grown with a normal B supply showed the strongest GUS activity in the steles, whereas after 3 d of -B treatment, GUS activity was elevated in the exodermis. After 6 d of -B treatment, GUS activity was again strong in the stele. Our results demonstrate that Os BOR1 is required both for efficient B uptake and for xylem loading of B. Possible roles of the temporal changes in tissue-specific patterns of Os BOR1 expression in response to B condition are discussed.
- Link information
-
- DOI
- https://doi.org/10.1105/tpc.106.049015
- CiNii Articles
- http://ci.nii.ac.jp/naid/80018194202
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17675406
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000249873800024&DestApp=WOS_CPL
- ID information
-
- DOI : 10.1105/tpc.106.049015
- ISSN : 1040-4651
- CiNii Articles ID : 80018194202
- Pubmed ID : 17675406
- Web of Science ID : WOS:000249873800024