2017年4月
Role of Thiolate Ligand in Spin State and Redox Switching in the Cytochrome P450 Catalytic Cycle
INORGANIC CHEMISTRY
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- 巻
- 56
- 号
- 8
- 開始ページ
- 4245
- 終了ページ
- 4248
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acs.inorgchem.6b02499
- 出版者・発行元
- AMER CHEMICAL SOC
The catalytic cycle of cytochrome P450 involves a change from the resting-state, water-bound, six coordinated form (1, low-spin state) to a five-coordinated form (2, high-spin state) upon binding of a hydrophobic substrate. Here, we used a heme thiolate model complex (SR complex) with THF as a model of nonionic H2O to address the question of whether or not coordination of nonionic water is sufficient to induce the low-spin state. Measurements of electronic absorption spectra and magnetic properties confirmed that five-coordinated SR complex has a high-spin state, and THF-bound, six coordinated SR has a low-spin state in dichloromethane at ambient temperature. The redox potential E-1/2 (Fe-II/F-III) of THF-bound SR was 80-90 mV more negative than that of five-coordinated SR. These properties indicate SR is a good model of P450. Our results suggest that thiolate coordination plays a key role in setting the low energy barrier between the high-spin and low-spin states.
- リンク情報
- ID情報
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- DOI : 10.1021/acs.inorgchem.6b02499
- ISSN : 0020-1669
- eISSN : 1520-510X
- PubMed ID : 28350457
- Web of Science ID : WOS:000399625600001