2014年2月
Whole Brain-Pituitary In Vitro Preparation of the Transgenic Medaka (Oryzias latipes) as a Tool for Analyzing the Differential Regulatory Mechanisms of LH and FSH Release
ENDOCRINOLOGY
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- 巻
- 155
- 号
- 2
- 開始ページ
- 536
- 終了ページ
- 547
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1210/en.2013-1642
- 出版者・発行元
- ENDOCRINE SOC
Two types of gonadotropins, luteinizing hormone (LH) and follicle stimulating hormone (FSH), are important pituitary hormones for sexual maturation and reproduction, and both of them are centrally regulated by gonadotropin-releasing hormone (GnRH) from the hypothalamus. In mammals, these two gonadotropins are secreted from a single type of gonadotrope. The mechanisms of differential regulation by GnRH of the release of two types of gonadotropins with different secretory profiles are still unknown. In teleosts, however, LH and FSH are secreted from separate cellular populations, unlike in mammals. This feature makes them useful for studying the regulatory mechanisms of LH and FSH secretions independently. Here, we generated transgenic medakalines that express Ca2+ indicator protein, inverse-pericam, specifically in theLHor FSH cells. We performed cell-type-specific Ca2+ imaging of LH and FSH cells, respectively, using the whole brain-pituitary preparations of these transgenic fish in which all neural circuits and GnRH neuronal projection to the pituitary are kept intact. LH and FSH cells showed different Ca2+ responses to GnRH. The results suggest differential regulation mechanisms for LH and FSH release by GnRH. Moreover, we also succeeded in detecting the effect on LH cells of endogenous GnRH peptide, which was released by electrical stimulation of the axons of GnRH1 neurons. Thus, our newly developed experimental model system using the whole brain-pituitary in vitro preparation of the transgenic medaka is a powerful tool for analyzing the differential regulatory mechanisms of the release of LH and FSH by multisynaptic neural inputs to the pituitary.
- リンク情報
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- DOI
- https://doi.org/10.1210/en.2013-1642
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702201582814457
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24248459
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000332382600019&DestApp=WOS_CPL
- URL
- http://orcid.org/0000-0002-3482-3051
- ID情報
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- DOI : 10.1210/en.2013-1642
- ISSN : 0013-7227
- eISSN : 1945-7170
- J-Global ID : 201702201582814457
- ORCIDのPut Code : 24939732
- PubMed ID : 24248459
- Web of Science ID : WOS:000332382600019
- ORCIDで取得されたその他外部ID : a:1:{i:0;a:1:{s:8:"other-id";s:19:"WOS:000332382600019";}}