2006年10月
Procedure for increasing the detection responses of estrogens in LC-MS based on introduction of a nitrobenzene moiety followed by electron capture atmospheric pressure chemical ionization
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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- 巻
- 386
- 号
- 3
- 開始ページ
- 658
- 終了ページ
- 665
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/s00216-006-0371-z
- 出版者・発行元
- SPRINGER HEIDELBERG
A practical procedure for determining estrogens in biological fluids has been studied using liquid chromatography-electron capture atmospheric pressure chemical ionization-mass spectrometry combined with derivatization. Among the commercially available reagents (4-nitrobenzoyl chloride, 2,4-dinitrofluorobenzene, 4-nitrobenzenesulfonyl chloride and 4-nitrobenzyl bromide), 4-nitrobenzenesulfonyl chloride was of the most practical use; it rapidly and quantitatively reacted with estrogens and increased the detection responses by 8-23 times. The derivatization method allowed the reproducible and accurate quantification of serum and urine estrone and estradiol of a pregnant woman, which is useful for diagnosis of the fetoplacental function, with small amounts (10 mu l) of sample and a simple pretreatment procedure.
- リンク情報
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- DOI
- https://doi.org/10.1007/s00216-006-0371-z
- CiNii Articles
- http://ci.nii.ac.jp/naid/80018920648
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16609843
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000240713500030&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1007/s00216-006-0371-z
- ISSN : 1618-2642
- CiNii Articles ID : 80018920648
- PubMed ID : 16609843
- Web of Science ID : WOS:000240713500030