2014年2月
Promotion of tracheal cartilage growth by intra-tracheal injection of basic fibroblast growth factor (b-FGF)
JOURNAL OF PEDIATRIC SURGERY
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- 巻
- 49
- 号
- 2
- 開始ページ
- 296
- 終了ページ
- 300
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jpedsurg.2013.11.040
- 出版者・発行元
- W B SAUNDERS CO-ELSEVIER INC
Purpose: Basic fibroblast growth factor (b-FGF) is a very effective growth factor that induces the proliferation of chondrocytes. This study aimed to investigate whether intra-tracheally-injected b-FGF solution promotes the growth of tracheal cartilage.
Methods: Group 1: 500 mu l of distilled water was injected at the posterior wall of the cervical trachea of New Zealand white rabbits by using a tracheoscope (n = 5). Group 2: 100 mu g/500 mu l of b-FGF solution was injected at the posterior wall of the cervical trachea (n = 5). Group 3: Biodegradable gelatin hydrogel microspheres incorporating 100 mu g/500 mu l of b-FGF solution were injected at the posterior wall of the cervical trachea (n = 5). All animals were sacrificed 4 weeks later, and the outer diameter and luminal area of the cervical trachea at the site of b-FGF injection were measured.
Results: The cervical tracheas in the two b-FGF injection groups were spindle-shaped and had a maximum diameter at the injection site. The median outer diameter of the cervical trachea in Groups 1, 2, and 3 was 7.3, 8.0, and 8.0 mm, respectively, showing a significant difference among Groups 1, 2, and 3 (P = 0.04). The median luminal area in Groups 1, 2, and 3 was 27.4, 29.4, and 32.1 mm(2), respectively. The ad hoc test showed a marginally significant difference only between groups 1 and 3 (p = 0.056).
Conclusion: Intra-tracheal injection of slowly released b-FGF enlarged the tracheal lumen. (C) 2014 Elsevier Inc. All rights reserved.
Methods: Group 1: 500 mu l of distilled water was injected at the posterior wall of the cervical trachea of New Zealand white rabbits by using a tracheoscope (n = 5). Group 2: 100 mu g/500 mu l of b-FGF solution was injected at the posterior wall of the cervical trachea (n = 5). Group 3: Biodegradable gelatin hydrogel microspheres incorporating 100 mu g/500 mu l of b-FGF solution were injected at the posterior wall of the cervical trachea (n = 5). All animals were sacrificed 4 weeks later, and the outer diameter and luminal area of the cervical trachea at the site of b-FGF injection were measured.
Results: The cervical tracheas in the two b-FGF injection groups were spindle-shaped and had a maximum diameter at the injection site. The median outer diameter of the cervical trachea in Groups 1, 2, and 3 was 7.3, 8.0, and 8.0 mm, respectively, showing a significant difference among Groups 1, 2, and 3 (P = 0.04). The median luminal area in Groups 1, 2, and 3 was 27.4, 29.4, and 32.1 mm(2), respectively. The ad hoc test showed a marginally significant difference only between groups 1 and 3 (p = 0.056).
Conclusion: Intra-tracheal injection of slowly released b-FGF enlarged the tracheal lumen. (C) 2014 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.jpedsurg.2013.11.040
- ISSN : 0022-3468
- eISSN : 1531-5037
- Web of Science ID : WOS:000331517600016