Recent developments in pharmaceutical technology allow systematic investigation of molecules operating in a living cell, facilitating identification of key players, which show potential as novel drug targets. One of the tools that has emerged for identifying potential antigens for use as vaccines against infectious diseases is time-course proteome analysis. The model virulent microorganism Candida albicans has been well studied in terms of both physiological characteristics and clinical aspects for developing pharmaceutics in treatments. In this article, we review a time-course proteome study of C. albicans during adaptation to a serum. Furthermore, we introduce a novel biotechnological strategy for developing vaccines using characteristic proteins that have been identified as virulence-related molecules. © 2014 Shibasaki S, et al.