1998年9月
Hydrophobilization of an esterase by genetic combination with polyproline at the carboxyl terminal
BIOTECHNOLOGY AND BIOENGINEERING
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- 巻
- 59
- 号
- 5
- 開始ページ
- 582
- 終了ページ
- 586
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/(SICI)1097-0290(19980905)59:5<582::AID-BIT8>3.0.CO;2-6
- 出版者・発行元
- WILEY-BLACKWELL
Polyproline, which is an amphiphilic polypeptide, was incorporated into the carboxyl terminal of an esterase by the recombinant DNA technique. The hydrophobicity of the esterase increased with increasing chain length of polyproline without inducing significant conformational changes. The mutant esterase catalyzed the hydrolysis of long-chain carboxylic acid ester more efficiently than the native esterase. It is considered that the alteration of substrate specificity is due to enhanced access of the mutant esterases to hydrophobic substrates. (C) 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59: 582-586, 1998.
- リンク情報
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- DOI
- https://doi.org/10.1002/(SICI)1097-0290(19980905)59:5<582::AID-BIT8>3.0.CO;2-6
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902158083046222
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/10099375
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000074997700008&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1002/(SICI)1097-0290(19980905)59:5<582::AID-BIT8>3.0.CO;2-6
- ISSN : 0006-3592
- eISSN : 1097-0290
- J-Global ID : 200902158083046222
- PubMed ID : 10099375
- Web of Science ID : WOS:000074997700008