2005年5月
Spatial distribution and functional significance of activated vinculin in living cells
JOURNAL OF CELL BIOLOGY
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- ,
- ,
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- 巻
- 169
- 号
- 3
- 開始ページ
- 459
- 終了ページ
- 470
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1083/jcb.200410100
- 出版者・発行元
- ROCKEFELLER UNIV PRESS
Conformational change is believed to be important to vinculin's function at sites of cell adhesion. However, nothing is known about vinculin's conformation in living cells. Using a Forster resonance energy transfer probe that reports on changes in vinculin's conformation, we find that vinculin is in the actin-binding conformation in a peripheral band of adhesive puncta in spreading cells. However, in fully spread cells with established polarity, vinculin's conformation is variable at focal adhesions. Time-lapse imaging reveals a gradient of conformational change that precedes loss of vinculin from focal adhesions in retracting regions. At stable or protruding regions, recruitment of vinculin is not necessarily coupled to the actin-binding conformation. However, a different measure of vinculin conformation, the recruitment of vinexin β by activated vinculin, shows that auto-inhibition of endogenous vinculin is relaxed at focal adhesions. Beyond providing direct evidence that vinculin is activated at focal adhesions, this study shows that the specific functional conformation correlates with regional cellular dynamics.
- リンク情報
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- DOI
- https://doi.org/10.1083/jcb.200410100
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902231850647600
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15883197
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000228995800012&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1083/jcb.200410100
- ISSN : 0021-9525
- J-Global ID : 200902231850647600
- PubMed ID : 15883197
- Web of Science ID : WOS:000228995800012