2011年3月
Production of prenylated flavonoids in tomato fruits expressing a prenyltransferase gene from Streptomyces coelicolor A3(2)
PLANT BIOLOGY
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- 巻
- 13
- 号
- 2
- 開始ページ
- 411
- 終了ページ
- 415
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1438-8677.2010.00409.x
- 出版者・発行元
- WILEY-BLACKWELL
Flavonoids are natural compounds found in many plants, including the important fruit crop, tomato. Prenylated flavonoids consist of a large group of compounds, which often exhibit antitumour, antibacterial and/or anti-androgen activities. In this study, we engineered the biosynthesis of prenylated flavonoids using a Streptomyces prenyltransferase HypSc (SCO7190) possessing broad-range substrate specificity, in tomato as a host plant. LC/MS/MS analysis demonstrated the generation of 3'-dimethylallyl naringenin in tomato fruits when recombinant HypSc protein was targeted to the plastids, whereas the recombinant protein hardly produced this compound in vitro. This is the first report confirming the accumulation of a prenylated flavonoid using a bacterial prenyltransferase in transgenic plants, and our results suggest that the product specificities of prenyltransferases can be significantly influenced by the host plant.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1438-8677.2010.00409.x
- ISSN : 1435-8603
- PubMed ID : 21309988
- Web of Science ID : WOS:000287198800021