Kazuhito Fujiyama

Profile Information

Affiliation: International Center for Biotechnology, Osaka University

Degree: Dr. of Engineering(Mar, 1990, Osaka University)

J-GLOBAL ID: 200901007092275232
researchmap Member ID: 1000030864

External link: http://www.icb.osaka-u.ac.jp/fujiyama_lab/index.html

Research Areas

Life sciences / Applied molecular and cellular biology /
Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering /

Research History

Feb, 2009 - Present

Professor, Osaka University International Center for Biotechnology
Feb, 2003 - Present

大阪大学助教授
Apr, 1988 - Present

Osaka University
Apr, 2007 - Jan, 2009

Associate Professor, Osaka University International Center for Biotechnology
Feb, 2003 - Mar, 2007

Associate Professor, Osaka University International Center for Biotechnology
Apr, 1995 - Jan, 2003

Research Assistant, Osaka University International Center for Biotechnology
1988 - 2003

Osaka University, Research Assistant

Education

- Mar, 1986

Graduate School, Division of Engineering, Osaka University
- 1986

Graduate School, Division of Engineering, Osaka University
- Mar, 1984

Faculty of Engineering, Osaka University
- 1984

Faculty of Engineering, Osaka University

Awards

Sep, 2019

日本生物工学会論文賞, 日本生物工学会

菅沼政俊・野村雄・比嘉友紀子・片岡由起子・船隈俊介・岡﨑博之・鈴木健夫・藤山和仁・瀬筒秀樹・立松謙一郎・田村俊樹
1999

日本生物工学会論文賞
1994

日本生物工学会論文賞

藤山和仁

Papers

134

Recent advances in plant-based bioproduction

Kazuhito Fujiyama, Toshiya Muranaka, Atsushi Okazawa, Hikaru Seki, Goro Taguchi, Shuhei Yasumoto

Journal of Bioscience and Bioengineering, Apr, 2024
Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana

Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama

Frontiers in Plant Science, 14, Aug 8, 2023
High accumulation of the Man5GlcNAc2 structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1

Keigo Sato, Hitomi Yumioka, Junko Isoyama, Koji Dohi, Akihiro Yamanaka, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering, 136(2) 136-141, Aug, 2023
High accumulation of the Man5GlcNAc2 structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1.

Keigo Sato, Hitomi Yumioka, Junko Isoyama, Koji Dohi, Akihiro Yamanaka, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Journal of bioscience and bioengineering, 136(2) 136-141, Aug, 2023
Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana.

Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama

Frontiers in plant science, 14 1215580-1215580, 2023
Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells

John Benson D. Choa, Tadahiro Sasaki, Hiroyuki Kajiura, Kazuyoshi Ikuta, Kazuhito Fujiyama, Ryo Misaki

Cytotechnology, 2023
Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems.

Hiroyuki Kajiura, Ken-Ichiro Tatematsu, Tsuyoshi Nomura, Mitsuhiro Miyazawa, Akihiro Usami, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama

Scientific reports, 12(1) 18502-18502, Nov 2, 2022
Impact of glycoengineering and antidrug antibodies on the anticancer activity of a plant-made lectin-Fc fusion protein.

Matthew Dent, Katarina L Mayer, Noel Verjan Garcia, Haixun Guo, Hiroyuki Kajiura, Kazuhito Fujiyama, Nobuyuki Matoba

Plant biotechnology journal, 20(11) 2217-2230, Nov, 2022
Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression.

Yotaro Yamamoto, Hiroyuki Kajiura, Takahiro Nishibu, Kazuhito Fujiyama

Journal of bioscience and bioengineering, 134(5) 432-440, Nov, 2022
High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves.

Edjohn Aaron Macauyag, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Plant biotechnology (Tokyo, Japan), 39(3) 291-301, Sep 25, 2022
Heterologous fusion gene expression and characterization of a novel carbohydrate binding module (Cbm36) to laccase (Lcc2)

Anita Kurniati, Ni Nyoman Tri Puspaningsih, Kartika Dwi Asni Putri, Mamik Damayanti, Ni Nyoman Purwani, Sylvia Aulia Rahmah, Purkan, Kazuhito Fujiyama, Makiko Sakka, Kazuo Sakka …

Biocatalysis and Agricultural Biotechnology, 42 102377-102377, Jul, 2022 Peer-reviewed
Production of recombinant β-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles

Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering, 133(5) 481-488, May, 2022 Peer-reviewedLast authorCorresponding author
Effect of fruit maturation on N-glycosylation of plant-derived native and recombinant miraculin

Hiroyuki Kajiura, Kyoko Hiwasa-Tanase, Hiroshi Ezura, Kazuhito Fujiyama

Plant Physiology and Biochemistry, 178 70-79, May, 2022 Peer-reviewed
Temporal analysis of N-acetylglucosamine extension of N-glycans in the middle silk gland of silkworm Bombyx mori.

Hiroyuki Kajiura, Takanori Eguchi, Keiro Uchino, Ken-Ichiro Tatematsu, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama

Journal of bioscience and bioengineering, 133(6) 533-540, Apr 6, 2022 Peer-reviewedLast authorCorresponding author
Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter.

Ryo Misaki, Masashi Iwasaki, Hiroki Takechi, Noriko Yamano-Adachi, Takao Ohashi, Hiroyuki Kajiura, Kazuhito Fujiyama

Cytotechnology, 74(1) 163-179, Feb, 2022 Peer-reviewedLast author
Biochemical characterization of Arabidopsis clade F polygalacturonase shows a substrate preference toward oligogalacturonic acids

Takao Ohashi, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering, 133(1) 1-7, Jan, 2022 Peer-reviewedLast author
Analysis of N-glycan profile of Arabidopsis alg3 cell culture.

Ratna Sariyatun, Hiroyuki Kajiura, Juthamard Limkul, Ryo Misaki, Kazuhito Fujiyama

Plant biotechnology (Tokyo, Japan), 38(4) 463-467, Dec 25, 2021
Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis

Hiroyuki Kajiura, Ryousuke Miyauchi, Akemi Kakudo, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Scientific Reports, 11(1), Dec, 2021 Peer-reviewedLast authorCorresponding author
Structure–function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis

Hiroyuki Kajiura, Takuya Yoshizawa, Yuji Tokumoto, Nobuaki Suzuki, Shinya Takeno, Kanokwan Jumtee Takeno, Takuya Yamashita, Shun-ichi Tanaka, Yoshinobu Kaneko, Kazuhito Fujiyama …

Communications Biology, 4(1), Dec, 2021 Peer-reviewed
Improved Assay System for Acidic Peptide: N-glycanase (aPNGase) Activity in Plant Extracts

Chiharu YAMAMOTO, Mikako OGURA, Ryota UEMURA, Megumi MAEDA, Hiroyuki KAJIURA, Ryo MISAKI, Kazuhito FUJIYAMA, Yoshinobu KIMURA

Anal Biochem., 114367-114367, Sep, 2021 Peer-reviewed

Misc.

An impact of cellular core-fucosylation in hepatitis B virus infection into hepatoma cell lines

Eiji Miyoshi, Shinji Takamatsu, Mayuka Shimomura, Yoshihiro Kamada, Haruka Maeda, Tomoaki Subajima, Hayato Hikita, Iijima Mayumi, Yuta Okamato, Ryo Misaki …

HEPATOLOGY, 62 1009A-1010A, Oct, 2015
Improvement of glycosylation structure by suppression of β-N-acetylglucosaminidases in silkworm

Nomura Tsuyoshi, Suganuma Masatoshi, Higa Yukiko, Kataoka Yukiko, Funaguma Shunsuke, Okazaki Hironobu, Suzuki Takeo, Kobayashi Isao, Sezutsu Hideki, Fujiyama Kazuhito

Journal of bioscience and bioengineering, 119(2) 131-136, Feb, 2015
1P-051 RasGRP1 and PLC-γ1 activate Ras on recycling endosomes

Matsumoto Kenji, Itokawa Kazuki, Mori Masahito, Misaki Ryo, Ohashi Takao, Fujiyama Kazuhito

67 102-102, 2015
3P-217 Novel L-Galactosyltransferase activity for elucidation of plant cell wall biosynthesis

Ohashi Hiroyuki, Ohashi Takao, Misaki Ryo, Kitamura Shinichi, Fujiyama Kazuhito

67 325-325, 2015
2P-275 Unravelling the N-glycan structures of some basidiomycetous yeasts in the class Microbotryomycetes (subphylum Pucciniomycotina, phylum Basidiomycota)

Ronilo Jose Danila Flores, Takao Ohashi, Hiroko Kawasaki, Kazuhito Fujiyama

67 243-243, 2015
2P-276 Glycan analysis of a pharmaceutical protein produced by transgenic silkworm

Sugimura Rie, Tatematsu Ken-ichiro, Sezutsu hideki, Misaki Ryo, Fujiyama Kazuhito

67 243-243, 2015
2P-279 Comparison of N-glycosylation patterns in Chinese Hamster Ovary K1 and DG44 cells

Maeda Akiko, Okamoto Yuta, Misaki Ryo, Ohashi Takao, Fujiyama Kazuhito

67 244-244, 2015
N-glycan sialylation in silkworm protein expression system and application

Yukiko Kataoka, Hironobu Okazaki, Tsuyoshi Nomura, Masatoshi Suganuma, Yukiko Higa, Shyunsuke Hunaguma, Takeo Suzuki, Akihiro Usami, Kazuhito Fujiyama, Hideki Sezutsu …

GLYCOBIOLOGY, 24(11) 1184-1184, Nov, 2014
1P-004 Optimization of transformation method in Rhodosporidium toruloides DMKU3-TK16 :

Tsai Yung-Yu, Ohashi Takao, Kanazwa Takenori, Polburee Pirapan, Misaki Ryo, Limtong Savitree, Fujiyama Kazuhito

66 18-18, 2014
3P-234 Characterization of Citrus maxima α-rhamnosyltransferase toward glycoside engineering

Hasegawa Yuka, Ohashi Takao, Misaki Ryo, Fujiyama Kazuhito

66 253-253, 2014
2P-256 Modification of plant glycosylation with glycosyltransferases fused to immunoglobulin-constant regions

Hadae Yukihiro, Misaki Ryo, Ohashi Takao, Fujiyama Kazuhito

66 170-170, 2014
3S-Ea04 Pharmaceutical protein production using heterologous host systems

Fujiyama Kazuhito

66 191-191, 2014
1P-225 Cloning and production of human anti-dengue virus antibodies

Masui Yukiko, Misaki Ryo, Sasaki Tadahiro, Pannamthip Pitaksajikul, Pongrama Ramasoota, Ikuta Kazuyoshi, Fujiyama Kazuhito

65 74-74, 2013
3P-240 Development of UDP-Rha production system using fission yeast

Hasegawa Yuka, Ohashi Takao, Fujiyama Kazuhito

65 248-248, 2013
3Ha11 Application of different 5' untranslated sequences for higher expression of human β-glucocerebrosidase in tobacco BY2 suspension culture :

Hong Nhung Ly, Kato Ko, Misaki Ryo, Kajiura Hiroyuki, Sasami Takahiro, Fujiyama Kazuhito

64 150-150, 2012
3Ha09 Construction and activity assay of chimeric glycosyltransferases fused to immunoglobulin-constant regions.

Hadae Yukihiro, Misaki Ryou, Fujiyama Kazuhito

64 150-150, 2012
3Ha08 Structural analysis of glycans produced in Schizosaccharomyces pombe α1,3-galactosyltransferase disruptants

Ohashi Hiroyuki, Ohashi Takao, Fujiyama Kazuhito

64 150-150, 2012
4Cp15 Identification of NeuAc synthetase from Clostridium kluyveri

Miyauchi Ryosuke, Kajiura Hiroyuki, Fujiyama Kazuhito

64 198-198, 2012
1Ep04 N-glycan structure analysis of Nicotiana tabacum SR1 grown in different temperature

Yamanaka Akihiro, Isoyama-Tanaka Junko, Dohi Koji, Misaki Ryo, Fujiyama Kazuhito

63 40-40, 2011
Recombinant expression and characterization of N-acetylglucosaminyltransferase I derived from Nicotiana tabacum

DOHI Koji, ISOYAMA-TANAKA Junko, TOKUDA Toru, FUJIYAMA Kazuhito

Journal of bioscience and bioengineering, 109(4) 388-391, Apr 25, 2010

Books and Other Publications

組換え医療用糖タンパク質の生産に向けた宿主細胞の育種

藤山和仁

酵素工学ニュース(酵素工学研究会), May, 2006
植物生産医療タンパク質と糖鎖

藤山和仁

日本生物工学会, Nov, 2005
植物バイオリアクターによる外来タンパク質生産特集によせて

藤山和仁

日本生物工学会, Nov, 2005
分子農業を目指した植物糖鎖工学

藤山和仁

財団法人バイオインダストリー協会, Dec, 2004
Pharmaceuticals in Plants in Encyclopedia of Plants and Crop Science

Kazuhito Fujiyama

Dekker Inc, Jan, 2004
The analysis of antigen-antibody bindiug using anti-RNaseA single chain Fv-3A21(共著)

Animal Cell Technology : Basic & Applied Aspects, 1994
Structure and expression of peroxidase isozyme genes in Horseradish and ┣DBArabidopsis(/)-┫DB

Plant Peroxidases : Biochemistry and Physiology University of Geneva, 1993
Structure of peroxidase isozyme genes in ┣DBBrassica(/)-┫DB and their expression

Biotechnology and Environmental Science : Molecular Approaches Plenum, Press, NY., 1992

Teaching Experience

2022

応用生物工学Ｂ
2022

先端バイオテクノロジー特論Ⅱ
2022

ESPバイオテクノロジーB
2022

基幹代謝学 II A
2022

基幹代謝学 II B
2022

学問への扉
2022

微生物学特論A
2022

微生物学特論B
2022

OUICP・産業バイオテクノロジーの基礎
2022

Combined Major Honor Seminar 5
2022

Combined Major Honor Seminar 4
2022

海外フィールドスタディS
2020

Field study program S "Bio-resource and Environment"
2020

Combined Major Honor Seminar 4
2020

Combined Major Honor Seminar 5
2020

OUICP-Fundamentals of Industrial Biotechnology
2020

Microbiology, Adv. B
2020

Microbiology, Adv. A
2020

A Door to Academia
2020

Reaction of Central Metabolism II-B

Professional Memberships

Works

Research Projects

Development of Escherichia coli capable of producing antibody with humanized glycan structure

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2018

Fujiyama Kazuhito
Development of fundamental technology to create E. coli carrying human-type glycosylation

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2013 - Mar, 2015

FUJIYAMA Kazuhito, OHASHI Takao
Thailand-Japan Collaboration for the Research and Development of Therapeutic Products against Infect

2012
Thailand-Japan Collaboration for the Research and Development of Therapeutic Products against Infect

2011
Next-genereation bioproduction platform leveraging subtropical microbial bioresources

2011
Thailand-Japan Collaboration for the Research and Development of Therapeutic Products against Infect

2010
Thailand-Japan Collaboration for the Research and Development of Therapeutic Products against Infect

2009
Thailand-Japan Collaboration for the Research and Development of Therapeutic Products against Infect

2008
Establishment of Bioproduction Research Center for Southeast Asian Bioresources

2007
Establishment of Bioproduction Research Center for Southeast Asian Bioresources

2006
De4veropment of rRNA database for yeast type strains and multiple gene analysis for yeast taxonomy

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2003 - 2005

SEKI Tatsuji, NAKAGAWA Hiroko, FUJIYAMA Kazuhito, SUGITA Takashi, HAMAMOTO Makiko, SUZUKI Motofumi
Plant vesicular transport engineering-molecular mechanisms of selective vesicular transport and application for recombinant protein production in plants

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2002 - 2004

YOSHIDA Kazuya, SHINMYO Atsuhiko, NAKAYAMA Hideki, SEKI Tatsuji, FUJIYAMA Kazuhito
植物におけるRNAiの機構解明と遺伝子制御への応用

2002
マメ科樹木由来の根粒形成細菌の多様性とその動態

科学研究費助成事業, 基盤研究(B), 日本学術振興会, 1998 - 2000

関達治, 冨田房男, 中川浩子, 藤山和仁, 阿部美紀子
食虫植物の有機化合物代謝機構の解析とその応用

科学研究費助成事業, 萌芽的研究, 日本学術振興会, 1997 - 1998

小林昭雄, 藤山和仁, 梶山慎一郎, 福崎英一郎
タバコ培養細胞によるヒト適応型糖鎖を持つタンパク質生産系開発のための細胞育種

科学研究費助成事業, 奨励研究(A), 日本学術振興会, 1997 - 1998

藤山和仁
Development of Fermentation Control Systems Applying Artificial Intelligent Techniques

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 1995 - 1996

YOSHIDA Toshiomi, NAKAJIMA Mikio, KISHIMOTO Michimasa, FUJIYAMA Kazuhito, TAKAGI Mutsumi, SEKI Tatsuji
Alcohol Production from Hemicellulosic Hydrolyszate by the Recombinant Yeast

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 1995 - 1996

YOSHIDA Toshiomi, TAKAGI Mutsumi, FUJIYAMA Kazuhito, SEKI Tatsuji
植物の糖鎖プロセシング機構に関する研究

1988
植物の酵素遺伝子のクローニング

1988

Industrial Property Rights

特開2002-238580 動物型糖鎖付加機能を持つ植物細胞

谷口直之, 関達治, 藤山和仁
特開2001-333787 動物型糖鎖付加機能をもつ植物細胞

谷口直之, 関達治, 藤山和仁

Academic Activities

International Workshop on Plant-Made-Pharmaceuticals

Planning, management, etc.

生物工学国際交流センター, Sep, 2014
日本におけるイタリア2011・秋

Other

イタリア政府, Nov, 2011
新シルクロード: カイコゲノムから新しい農業へ

Other

Oct, 2010
「21 世紀最大の未利用資源活用のための昆虫・テクノロジー研究」プロジェクトシンポジウム

Other

独立行政法人農業生物資源研究所, Sep, 2006
第24回日本植物細胞分子生物学会(つくば)大会・シンポジウム

Other

日本植物細胞分子生物学会, Jul, 2006
日本農芸化学会2006年度大会（北海道）「植物グリコミクスの鳥瞰と展望」

Other

日本農芸化学会, Mar, 2006
糖鎖工学センターGGプロジェクトセミナー

Other

糖鎖工学センター, Jan, 2004
国立感染症研究所学友会シンポジウム「QOL向上に貢献する植物科学への招待」

Other

国立感染症研究所学友会, Nov, 2001

Profile Information

Research Areas

Research History

Education

Awards

Papers

Misc.

Books and Other Publications

Teaching Experience

Professional Memberships

Works

Research Projects

Industrial Property Rights

Academic Activities

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