2009年4月
A centromeric DNA sequence colocalized with a centromere-specific histone H3 in tobacco
CHROMOSOMA
- ,
- ,
- 巻
- 118
- 号
- 2
- 開始ページ
- 249
- 終了ページ
- 257
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/s00412-008-0193-1
- 出版者・発行元
- SPRINGER
Centromeres play an important role in segregating chromosomes into daughter cells, and centromeric DNA assembles specific proteins to form a complex referred to as the kinetochore. Among these proteins, centromere-specific histone H3 (CENH3) is one of the most characterized and found to be located only on active centromeres. We isolated four different CENH3-coding complementary DNAs (cDNAs), two from Nicotiana tabaccum and one each from the ancestral diploid species, Nicotiana sylvestris and Nicotiana tomentosiformis and raised an antibody against N-terminal amino acid sequences deduced from the cDNAs. Immunostaining with the antibody revealed the preferential centromere localization, indicating that the cDNAs cloned in this study encode authentic tobacco CENH3. A tobacco centromeric DNA sequence (Nt2-7) was also identified by chromatin immunoprecipitation cloning using the antibody.
- リンク情報
- ID情報
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- DOI : 10.1007/s00412-008-0193-1
- ISSN : 0009-5915
- Web of Science ID : WOS:000264092600009