1996年
Pulmonary surfactant migrates into the alveolar capillaries of newborn rats: An immunoelectron microscopic study
ACTA ANATOMICA
- ,
- ,
- ,
- 巻
- 156
- 号
- 1
- 開始ページ
- 11
- 終了ページ
- 21
- 記述言語
- 英語
- 掲載種別
- 出版者・発行元
- KARGER
The goal of the present study was to elucidate the migration of surfactant-like material into the alveolar capillaries of newborn rats. The lungs of experimental animals were studied by ultrastructural techniques - standard electron microscopy as well as immunoelectron microscopy - using antibodies against synthetic polypeptide complementary to the noncollagenous part of surfactant protein A (SP-A). Antibodies exhibited specific reaction with native rat SP-A in Western blot and immunohistochemical analysis. Surfactant was observed in the alveolar space, type II pneumocytes, macrophages, type I pneumocytes, endothelial cells, the extracellular space of the alveolar septa and in the alveolar capillaries. Lamellar structures observed in all elements of the blood-air barrier as well as in the alveolar capillaries were stained positively with anti-SP-A antibodies. Transport of the pulmonary surfactant into the circulatory system was observed more frequently during the first 6-8 h of extrauterine Life of newborn rats, although it was also seen in the blood vessels till 48 h after delivery. The results of our investigation suggest that clearance of surfactant via the circulatory system plays an important role in the early postnatal period.
- リンク情報
- ID情報
-
- ISSN : 0001-5180
- Web of Science ID : WOS:A1996VT84900003