2002年6月
Two low-temperature-inducible Chlorella genes for Delta 12 and omega-3 fatty acid desaturase (FAD): Isolation of Delta 12 and omega-3 fad cDNA clones, expression of Delta 12 fad in Saccharomyces cerevisiae, and expression of omega-3 fad in Nicotiana tabacum
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- 巻
- 66
- 号
- 6
- 開始ページ
- 1314
- 終了ページ
- 1327
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1271/bbb.66.1314
- 出版者・発行元
- TAYLOR & FRANCIS LTD
In an attempt to clarify the involvement of fatty acid desaturases (FADs) in the freezing tolerance of Chlorella vulgaris IAM C-27, developed by hardening, we have isolated cDNA clones for two types of FADs from the Chlorella strain, based on the sequence information of genes for Delta12 and omega-3 FADs, respectively desaturating oleic acid (18:1) to linoleic acid (18:2) and linoleic acid (18:2) to linolenic acid (18:3). The deduced amino acid sequence of the first clone, designated CvFad2, showed about 66% similarity to the microsomal Delta12 FADs from several higher plants and this gene had Delta12 FAD activity when expressed in Saccharomyces cerevisiae. The predicted protein encoded by a second gene, designated CvFad3, showed about 60% similarity to the microsomal and plastidial omega-3 FADs from several higher plants. The features of the amino acid sequences of the C- and N-terminal regions of CvFAD3 and fatty acid analysis of polar lipids in transgenic tobacco plant expressing the CvFad3 gene suggested that this gene encodes the microsomal omega-3 FAD. Southern blot analysis showed that both genes were single-copy genes in the genome of the Chlorella strain. Different transcriptional patterns were observed with the two genes during hardening in Northern blot analysis.
- リンク情報
-
- DOI
- https://doi.org/10.1271/bbb.66.1314
- CiNii Articles
- http://ci.nii.ac.jp/naid/110002693822
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/12162554
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000176603800018&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1271/bbb.66.1314
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 110002693822
- PubMed ID : 12162554
- Web of Science ID : WOS:000176603800018