2016年12月1日
Restorative effect of organic germanium compound (Ge-132) on dermal injury
Wound Medicine
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- 巻
- 15
- 号
- 開始ページ
- 6
- 終了ページ
- 10
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.wndm.2016.09.001
- 出版者・発行元
- Elsevier GmbH
We investigated the effect of organic germanium compound (Ge-132) on experimental dermal injury in rats and cultured human dermal fibroblasts cells. For in vivo studies, the dermal wound was formed by extracting a full-thickness skin of parietal region in rats. The dermal wound was treated with 250 μl of 1.8% Ge-132 isotonic solution or physiological saline solution as a control once a day. At 1, 3, 7, and 14 days, the area of wound was measured and the dermal wound part was subjected to the histopathological examination. For in vitro studies, Hs68 cells were treated with Ge-132 (0.1–10 μg/ml) for 24 h, and then wound contraction was measured by collagen gel contraction assay and TGF-β1 and α-SMA mRNA expressions were measured by RT-PCR. A group without the addition of Ge-132 served as a control. We found that Ge-132 significantly reduced the wound area at 7 (P <
0.01) and 14 days (P <
0.05), and also decreased edema and increased proliferation of fibroblasts and formation of collagen fibers at 7 days, and induced necrosis, neutrophil infiltration, and macrophage at 14 days on experimental dermal injury in rats. In addition, Ge-132 increased gel contraction (P <
0.05), and TGF-β1 and α-SMA mRNA expressions (P <
0.05) at 24 h in Hs68 cell. These results suggested that Ge-132 might be effective in dermal wound healing.
0.01) and 14 days (P <
0.05), and also decreased edema and increased proliferation of fibroblasts and formation of collagen fibers at 7 days, and induced necrosis, neutrophil infiltration, and macrophage at 14 days on experimental dermal injury in rats. In addition, Ge-132 increased gel contraction (P <
0.05), and TGF-β1 and α-SMA mRNA expressions (P <
0.05) at 24 h in Hs68 cell. These results suggested that Ge-132 might be effective in dermal wound healing.
- ID情報
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- DOI : 10.1016/j.wndm.2016.09.001
- ISSN : 2213-9109
- ISSN : 2213-9095
- SCOPUS ID : 84988920775