Materals extracted grom GOISHICHA with water and ethyl alcohol were administered to rats at constant dose over a specified period and examination was made as to the manner in which fatty acids in each lipid fraction of rat brain and liver were affected. Brain weight in the case of material extraction with water (W-E) was found greater compared to extraction with ethyl alcohol (EA-E) though with no statistically significant difference. Total lipid (TG) was generally less in the EA-Egroup, while compound lipids (CL), less in the W-E group. Brain fatty acids C18:ln-9 of neutral lipids (NL) were abundantly present in the W-E and EA-E groups, with statistically significant increase being noted in the EA-E group. CL in C18:2n-6 was high in the W-E and EA-E groups and NL in C20:4n-6 was higher, and noted to significantly increase in the Ea-E group. Liver weight increased in the W-E and EA-E groups. Compound lipids (CL) were present in the EA-E group. C18:3n-3 NL and CL were greater in the EA-E group, statistically significantly so in the latter. The present findings thus clearly indicate the extracts examined here to significantly brain fatty acid levels. © 2006, Japan Oil Chemists' Society. All rights reserved.